J Bacteriol. 2005 Mar;187(5):1581-90. Experimental identification and quantification of glucose metabolism in seven bacterial species. Fuhrer T, Fischer E, Sauer U.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 254.24 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 105.81 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 151.63 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 43.82 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 150.14 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 36.17 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 163.61 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 46.15 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 233.87 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 87.1 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 271.79 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 110.79 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 316.53 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 136.53 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 1.64 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| Growth Condition | |
| Strains | Agrobacterium tumefaciens C58 |
| Genotype | |
| Culture medium | The M9 minimal medium contained, per liter of deionized water, 7.52 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 0.5 g of NaCl,and 2.5 g of (NH4)2SO4.The following components (in indicated quantities per liter of final medium) were sterilized separately and then added: 1 mL of 0.1 M CaCl2, 1 mL of 1M MgSO4, 0.6 mL of 100 mM FeCl3, 2 ml of vitamin solution (filter sterilized),and 10 mL of M9 trace salts solution. The vitamin solution contained (per 50 ml) 25 mg each of biotin, cyanocobalamin, niacin, calcium pantothenate, pyridoxine HCl,and thiamine HCl.The M9 trace salts solution contained (per liter) 0.18 g of ZnSO4-7H2O,0.12 g of CuCl2-2H2O,0.12 g of MnSO4-H2O,and 0.18 g of CoCl2-6H2O. |
| Carbon source | Glucose |
| Growth rate | 0.3/h |
| Case-specific description | In vivo carbon flux distribution in A.tumefaciens. The fluxes were normalized to the glucose uptake rate of 4.5 mmol/g/h. |
| Growth Condition | |
| Strains | Bacillus subtilis 168 trpC2 |
| Genotype | |
| Culture medium | The M9 minimal medium contained, per liter of deionized water, 7.52 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 0.5 g of NaCl,and 2.5 g of (NH4)2SO4.The following components (in indicated quantities per liter of final medium) were sterilized separately and then added: 1 mL of 0.1 M CaCl2, 1 mL of 1M MgSO4, 0.6 mL of 100 mM FeCl3, 2 ml of vitamin solution (filter sterilized),and 10 mL of M9 trace salts solution. The vitamin solution contained (per 50 ml) 25 mg each of biotin, cyanocobalamin, niacin, calcium pantothenate, pyridoxine HCl,and thiamine HCl.The M9 trace salts solution contained (per liter) 0.18 g of ZnSO4-7H2O,0.12 g of CuCl2-2H2O,0.12 g of MnSO4-H2O,and 0.18 g of CoCl2-6H2O. |
| Carbon source | Glucose |
| Growth rate | 0.30/h |
| Case-specific description | In vivo carbon flux distribution in B.subtilis. The fluxes were normalized to the glucose uptake rate of 4.6 mmol/g/h. |
| Growth Condition | |
| Strains | Escherichia coli MG1655 |
| Genotype | |
| Culture medium | The M9 minimal medium contained, per liter of deionized water, 7.52 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 0.5 g of NaCl,and 2.5 g of (NH4)2SO4.The following components (in indicated quantities per liter of final medium) were sterilized separately and then added: 1 mL of 0.1 M CaCl2, 1 mL of 1M MgSO4, 0.6 mL of 100 mM FeCl3, 2 ml of vitamin solution (filter sterilized),and 10 mL of M9 trace salts solution. The vitamin solution contained (per 50 ml) 25 mg each of biotin, cyanocobalamin, niacin, calcium pantothenate, pyridoxine HCl,and thiamine HCl.The M9 trace salts solution contained (per liter) 0.18 g of ZnSO4-7H2O,0.12 g of CuCl2-2H2O,0.12 g of MnSO4-H2O,and 0.18 g of CoCl2-6H2O. |
| Carbon source | Glucose |
| Growth rate | 0.39/h |
| Case-specific description | In vivo carbon flux distribution in E.coli. The fluxes were normalized to the glucose uptake rate of 7.5 mmol/g/h. |
| Growth Condition | |
| Strains | Pseudomonas fluorescens 52-1C |
| Genotype | |
| Culture medium | The M9 minimal medium contained, per liter of deionized water, 7.52 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 0.5 g of NaCl,and 2.5 g of (NH4)2SO4.The following components (in indicated quantities per liter of final medium) were sterilized separately and then added: 1 mL of 0.1 M CaCl2, 1 mL of 1M MgSO4, 0.6 mL of 100 mM FeCl3, 2 ml of vitamin solution (filter sterilized),and 10 mL of M9 trace salts solution. The vitamin solution contained (per 50 ml) 25 mg each of biotin, cyanocobalamin, niacin, calcium pantothenate, pyridoxine HCl,and thiamine HCl.The M9 trace salts solution contained (per liter) 0.18 g of ZnSO4-7H2O,0.12 g of CuCl2-2H2O,0.12 g of MnSO4-H2O,and 0.18 g of CoCl2-6H2O. |
| Carbon source | Glucose |
| Growth rate | 0.49/h |
| Case-specific description | In vivo carbon flux distribution in P.fluorescens. The fluxes were normalized to the glucose uptake rate of 4.5 mmol/g/h. |
| Growth Condition | |
| Strains | Rhodobacter sphaeroides ATH2.4.1 |
| Genotype | |
| Culture medium | The M9 minimal medium contained, per liter of deionized water,7.52 g of Na2HPO4-2H2O,3.0 g of KH2PO4, 0.5 g of NaCl,and 2.5 g of (NH4)2SO4.The following components (in indicated quantities per liter of final medium) were sterilized separately and then added: 1 mL of 0.1 M CaCl2, 1 mL of 1 M MgSO4, 0.6 mL of 100 mM FeCl3, 2 ml of vitamin solution (filter sterilized),and 10 mL of M9 trace salts solution. The vitamin solution contained (per 50 ml) 25 mg each of biotin, cyanocobalamin, niacin, calcium pantothenate, pyridoxine HCl,and thiamine HCl.For R.sphaeroides, we used a special trace salts solution that contained (per liter) 1.5 g of nitrilotriacetic acid, 3.0 g of MgSO4-7H2O, 0.5 g of MnSO4-H2O, 1.0 g of NaCl, 0.1 g of FeSO4-7H2O, 0.1 g of CoCl2-6H2O, 0.135 g of CaCl2-2H2O, 0.1 g of ZnSO4-7H2O, 0.01 g of CuSO4-5H2O, 0.01 g of H3BO3, 0.01 g of Na2MoO4-2H2O, 0.015 g of NiCl2,and 0.02 g of Na2SeO3;the pH was adjusted to 6.5 with KOH. |
| Carbon source | Glucose |
| Growth rate | 0.15/h |
| Case-specific description | In vivo carbon flux distribution in R.sphaeroides. The fluxes were normalized to the glucose uptake rate of 1.9 mmol/g/h. |
| Growth Condition | |
| Strains | Sinorhizobium meliloti |
| Genotype | |
| Culture medium | The M9 minimal medium contained, per liter of deionized water, 7.52 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 0.5 g of NaCl,and 2.5 g of (NH4)2SO4.The following components (in indicated quantities per liter of final medium) were sterilized separately and then added: 1 mL of 0.1 M CaCl2, 1 mL of 1M MgSO4, 0.6 mL of 100 mM FeCl3, 2 ml of vitamin solution (filter sterilized),and 10 mL of M9 trace salts solution. The vitamin solution contained (per 50 ml) 25 mg each of biotin, cyanocobalamin, niacin, calcium pantothenate, pyridoxine HCl,and thiamine HCl.The M9 trace salts solution contained (per liter) 0.18 g of ZnSO4-7H2O,0.12 g of CuCl2-2H2O,0.12 g of MnSO4-H2O,and 0.18 g of CoCl2-6H2O. |
| Carbon source | Glucose |
| Growth rate | 0.17/h |
| Case-specific description | In vivo carbon flux distribution in S.meliloti. The fluxes were normalized to the glucose uptake rate of 2.4 mmol/g/h. |
| Growth Condition | |
| Strains | Paracoccus versutus A2 |
| Genotype | |
| Culture medium | The Paracoccus versutus minimal medium was composed of two solutions that were mixed at a ratio of 1:10 after heat sterilization. Solution A contained (per 100mL) 4.2 g of Na2HPO4-2H2O,1.5 g of KH2PO4,and 1.0 g of NH4Cl;the pH was adjusted to 9.0.Solution B contained (per liter) 0.1 g of MgSO4-7H2O and 5.0 mL of trace metal solution;the pH was adjusted to 6.0 with KOH. The trace solution contained (per liter) 50.0 g of EDTA, 22.0 g of ZnSO4-7H2O, 5.54 g of CaCl2-2H2O, 5.06 g of MnCl2-4H2O, 4.99 g of FeSO4-7H2O, 1.10 g of MoNH4-4H2O, 1.57 g of CuSO4-5H2O,and 1.61 g of CoCl2-6H2O. |
| Carbon source | Glucose |
| Growth rate | 0.70/h |
| Case-specific description | In vivo carbon flux distribution in P.versutus. The fluxes were normalized to the glucose uptake rate of 18.1 mmol/g/h. |
| Growth Condition | |
| Strains | Zymomonas mobilis NRRL B-806 |
| Genotype | |
| Culture medium | The Z.mobilis minimal medium contained (per liter) 0.18 g of KH2PO4, 0.082 g of MgSO4-7H2O, 0.002 g of FeSO4-7H2O, 0.87 g of NH4Cl, 0.142 g of trisodium citrate dihydrate, 10 g of potassium hydrogen phthalate,and 2 ml of the vitamin solution (filter sterilized).The pH was adjusted to 5.8 with KOH. |
| Carbon source | Glucose |
| Growth rate | 0.34/h |
| Case-specific description | In vivo carbon flux distribution in Z.mobilis. The fluxes were normalized to the glucose uptake rate of 61.5 mmol/g/h. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 4.1 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 4.8 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 7.8 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 4.5 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 1.8 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 2.3 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 18.9 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 61.5 |