J Biotechnol. 2013 Dec;168(4):355-61. Metabolic flux analysis of Arthrobacter sp. CGMCC 3584 for cAMP production based on 13C tracer experiments and gas chromatography-mass spectrometry. Niu H, Chen Y, Yao S,et al.
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| ATP production rate Maximum | 699.25 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 497.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 125.3 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 44.6 | R02736+R01528 |
| ATP production rate Maximum | 698.15 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 500.4 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 135.3 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 56.2 | R02736+R01528 |
| ATP production rate Maximum | 634.1 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 462.6 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 125.2 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 56.6 | R02736+R01528 |
| Growth Condition | |
| Strains | Arthrobacter sp. CGMCC3584 |
| Genotype | |
| Culture medium | The slant and seed media and their culture conditions were as described by Chen et al.(2010). The fermentation medium contained(g/L): urea 8, K2HPO4 18, KH2PO4 5, MgSO4 0.1, biotin 0.01, and CoCl2 0.005. NaF(0.4 g/L) and HX(8 g/L) (used as a control) were added into the media. The initial pH was adjusted to 7.0 by NaOH and the medium was autoclaved for 20 min at 115 celsius. |
| Carbon source | Glucose. |
| Growth rate | 0.122/h |
| Case-specific description | Metabolic flux distributions of Arthrobacter sp.CGMCC3584 cultured in control medium. |
| Growth Condition | |
| Strains | Arthrobacter sp. CGMCC3584 |
| Genotype | |
| Culture medium | The slant and seed media and their culture conditions were as described by Chen et al.(2010). The fermentation medium contained(g/L): urea 8, K2HPO4 18, KH2PO4 5, MgSO4 0.1, biotin 0.01, and CoCl2 0.005. NaF(0.4 g/L) were added into the media. The initial pH was adjusted to 7.0 by NaOH and the medium was autoclaved for 20 min at 115 celsius. |
| Carbon source | Glucose. |
| Growth rate | 0.139/h |
| Case-specific description | Metabolic flux distributions of Arthrobacter sp.CGMCC3584 cultured in medium with NaF. |
| Growth Condition | |
| Strains | Arthrobacter sp. CGMCC3584 |
| Genotype | |
| Culture medium | The slant and seed media and their culture conditions were as described by Chen et al.(2010). The fermentation medium contained(g/L): urea 8, K2HPO4 18, KH2PO4 5, MgSO4 0.1, biotin 0.01, and CoCl2 0.005. HX (8 g/L) were added into the media. The initial pH was adjusted to 7.0 by NaOH and the medium was autoclaved for 20 min at 115 celsius. |
| Carbon source | Glucose. |
| Growth rate | 0.15/h |
| Case-specific description | Metabolic flux distributions of Arthrobacter sp.CGMCC3584 cultured in medium with HX. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 2.661 |
| Lactate | 0.292 |
| Acetate | 0.618 |
| 2-Oxoglutarate | 0.171 |
| Citrate | 0.211 |
| Pyruvate | 0.502 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 2.839 |
| Lactate | 0.335 |
| Acetate | 0.854 |
| 2-Oxoglutarate | 0.104 |
| Citrate | 0.334 |
| Pyruvate | 0.069 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 2.783 |
| Lactate | 0.271 |
| Acetate | 0.678 |
| 2-Oxoglutarate | 0.122 |
| Citrate | 0.191 |
| Pyruvate | 0.202 |