J Biosci Bioeng. 2014 Aug 13. pii: S1389-1723(14)00230-8. Comparative metabolic flux analysis of an Ashbya gossypii wild type strain and a high riboflavin-producing mutant strain. Jeong BY, Wittmann C, Kato T, et al.
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| ATP production rate Maximum | 2078.7 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 1444.45 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 314.3 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 60.6 | R02736+R01528 |
| ATP production rate Maximum | 1707.3 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 1214.8 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 275.4 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 78.4 | R02736+R01528 |
| Growth Condition | |
| Strains | Ashbya gossypii ATCC 10895 |
| Genotype | Wild type. |
| Culture medium | YD medium (pH 6.8) containing 10 g/L yeast extract and 10 g/L glucose was used for glycerol stocks. The seed culture and preculture were maintained in complex medium containing 20 g/L glucose, 25 g/L corn steep liquor, 1.0 g/L K2HPO4, and 5.0 g/L peptone.The main cultivation was performed in chemically defined minimal medium (pH 6.8) containing 50 mM glucose as a carbon source, 1.5 g/L asparagine, 0.75 g/L KH2PO4, 0.1 g/L myo-inositol, and mineral ions (4.4 mg/L CoCl2-6H2O, 18.0 mg/L MnCl2-4H2O, 44.0 mg/L ZnSO4-7H2O, 10.1 mg/L MgSO4-7H2O, 27.0 mg/L FeCl3-6H2O, 21.9 mg/L CaCl2-6H2O, and 2.7 mg/L CuSO4-5H2O). The stock solution for the mineral ions was autoclaved separately for 20 min at 121 celsius and cooled to room temperature prior to addition. |
| Carbon source | Glucose. |
| Growth rate | 1.014/h |
| Case-specific description | In vivocarbonflux distribution in the central metabolic pathway of wild type strains. |
| Growth Condition | |
| Strains | Ashbya gossypii W122032 |
| Genotype | |
| Culture medium | YD medium (pH 6.8) containing 10 g/L yeast extract and 10 g/L glucose was used for glycerol stocks. The seed culture and preculture were maintained in complex medium containing 20 g/L glucose, 25 g/L corn steep liquor, 1.0 g/L K2HPO4, and 5.0 g/L peptone.The main cultivation was performed in chemically defined minimal medium (pH 6.8) containing 50 mM glucose as a carbon source, 1.5 g/L asparagine, 0.75 g/L KH2PO4, 0.1 g/L myo-inositol, and mineral ions (4.4 mg/L CoCl2-6H2O, 18.0 mg/L MnCl2-4H2O, 44.0 mg/L ZnSO4-7H2O, 10.1 mg/L MgSO4-7H2O, 27.0 mg/L FeCl3-6H2O, 21.9 mg/L CaCl2-6H2O, and 2.7 mg/L CuSO4-5H2O). The stock solution for the mineral ions was autoclaved separately for 20 min at 121 celsius and cooled to room temperature prior to addition. |
| Carbon source | Glucose. |
| Growth rate | 0.783/h |
| Case-specific description | In vivocarbonflux distribution in the central metabolic pathway of high RIB-producing A.gossypii strains. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 0.899 |
| asparagine | 0.63 |
| riboflavin | 0.001 |
| lactate | 0.034 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 0.518 |
| asparagine | 0.097 |
| riboflavin | 0.005 |
| lactate | 0.069 |