Metab Eng. 2012 Jan;14(1):47-58. Integration of in vivo and in silico metabolic fluxes for improvement of recombinant protein production. Driouch H, Melzer G, Wittmann C.
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| ATP production rate Maximum | 849 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 656.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 129 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 52 | R02736+R01528 |
| ATP production rate Maximum | 225 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 120 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 122 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 80 | R02736+R01528 |
| Growth Condition | |
| Strains | Aspergillus niger SKANip8 |
| Genotype | Wild type. |
| Culture medium | The cultivation medium contained per litre: 15g glucose, 20mL salt solution (50* with 180g/L NaNO3, 10g/L KCl, 30g/L KH2PO4, 10g/L MgSO4-7H2O) and 1mL trace element solution (1000* with 10 g/L EDTA, 4.4g/L ZnSO4-7H2O, 1.5g/L MnCl2-4H2O, 0.32g/L CuSO4-5H2O, 7.5g/L FeSO4-7H2O,0.32g/L CoCl2-6H2O, 1.47g/L CaCl2-2H2O and 0.22g/L (NH4)6Mo7O24-4H2O). |
| Carbon source | Glucose |
| Growth rate | 0.17/h |
| Case-specific description | Aspergillus niger SKANip8 was cultivated on glucose minimal medium. |
| Growth Condition | |
| Strains | A. niger SKAn1015 |
| Genotype | The recombinant fructofuranosidase producer expresses the suc1 gene, encoding fructofuranosidase,under the control of the constitutive pkiA (pyruvate kinase) promoter. |
| Culture medium | The cultivation medium contained per litre: 15g glucose, 20mL salt solution (50* with 180g/L NaNO3, 10g/L KCl, 30g/L KH2PO4, 10g/L MgSO4-7H2O) and 1mL trace element solution (1000* with 10 g/L EDTA, 4.4g/L ZnSO4-7H2O, 1.5g/L MnCl2-4H2O, 0.32g/L CuSO4-5H2O, 7.5g/L FeSO4-7H2O,0.32g/L CoCl2-6H2O, 1.47g/L CaCl2-2H2O and 0.22g/L (NH4)6Mo7O24-4H2O). |
| Carbon source | Glucose |
| Growth rate | 0.17/h |
| Case-specific description | A. niger SKAn1015 was cultivated on glucose minimal medium. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.13 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.17 |