Bioprocess Biosyst Eng. 2007 Jan;30(1):47-59. Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates. Furch T, Hollmann R, Wittmann C, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 96 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 96 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 106 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 94 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 130 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 32 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 80 | R02736+R01528 |
| Growth Condition | |
| Strains | B. megaterium MS941 |
| Genotype | The delta nprM mutant lacks a major extracellular protease (NprM) which was derived by gene replacement. |
| Culture medium | For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution. |
| Carbon source | Glucose |
| Growth rate | 0.11/h |
| Case-specific description | Metabolic flux distribution of B.megaterium MS941 strain at C-limited cultivation conditions with the glucose uptake rate of 0.11/h. |
| Growth Condition | |
| Strains | B. megaterium MS941 |
| Genotype | The delta nprM mutant lacks a major extracellular protease (NprM) which was derived by gene replacement. |
| Culture medium | For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution. |
| Carbon source | Glucose |
| Growth rate | 0.426/h |
| Case-specific description | Metabolic flux distribution of B.megaterium MS941 strain at C-limited cultivation conditions with the glucose uptake rate of 0.426/h. |
| Growth Condition | |
| Strains | B. megaterium MS941 |
| Genotype | The delta nprM mutant lacks a major extracellular protease (NprM) which was derived by gene replacement. |
| Culture medium | For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution. |
| Carbon source | Glucose |
| Growth rate | 0.11/h |
| Case-specific description | Metabolic flux distribution of B.megaterium MS941 strain at C-limited cultivation conditions with the glucose uptake rate of 0.11/h(second run). |
| Growth Condition | |
| Strains | B. megaterium MS941 |
| Genotype | The delta nprM mutant lacks a major extracellular protease (NprM) which was derived by gene replacement. |
| Culture medium | For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution. |
| Carbon source | Glucose |
| Growth rate | 0.108/h |
| Case-specific description | Metabolic flux distribution of B.megaterium MS941 strain at N-limited cultivation conditions with the glucose uptake rate of 0.108/h. |
| Growth Condition | |
| Strains | B. megaterium DSM319 |
| Genotype | Parental strain. |
| Culture medium | For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution. |
| Carbon source | Glucose |
| Growth rate | 0.106/h |
| Case-specific description | Metabolic flux distribution of B.megaterium DSM319 strain at C-limited cultivation conditions with the glucose uptake rate of 0.106/h. |
| Growth Condition | |
| Strains | B. megaterium WH320 |
| Genotype | WH320, a delta lacZ mutant of DSM319, was received by chemical mutagenesis using ethyl methanesulfonate. |
| Culture medium | For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution. |
| Carbon source | Glucose |
| Growth rate | 0.096/h |
| Case-specific description | Metabolic flux distribution of B.megaterium WH320 strain at C-limited cultivation conditions with the glucose uptake rate of 0.096/h. |
| Growth Condition | |
| Strains | B. megaterium WH323 |
| Genotype | The xylose-deficient strain WH323 was derived from WH320 by inserting the lacZ gene into the xylA locus of WH320. |
| Culture medium | For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution. |
| Carbon source | Glucose |
| Growth rate | 0.107/h |
| Case-specific description | Metabolic flux distribution of B.megaterium WH323 strain at C-limited cultivation conditions with the glucose uptake rate of 0.107/h. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.62 |
| CO2 | 4.48 |
| acetate | 0.17 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 5.17 |
| CO2 | 11.61 |
| acetate | 0.6 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.58 |
| CO2 | 4.52 |
| acetate | 0.15 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.47 |
| CO2 | 4.19 |
| acetate | 0.13 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.52 |
| CO2 | 4.6 |
| acetate | 0.15 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.31 |
| CO2 | 4 |
| acetate | 0.17 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.53 |
| CO2 | 3.9 |
| acetate | 0.16 |