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Bioprocess Biosyst Eng. 2007 Jan;30(1):47-59. Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates. Furch T, Hollmann R, Wittmann C, et al.



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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 59 R02736+R01528+R00267+R00214
NADPH production rate Minium 96 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 99 R02736+R01528+R00267+R00214
NADPH production rate Minium 96 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 76 R02736+R01528+R00267+R00214
NADPH production rate Minium 106 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 54 R02736+R01528+R00267+R00214
NADPH production rate Minium 94 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 78 R02736+R01528+R00267+R00214
NADPH production rate Minium 130 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum -52 R02736+R01528+R00267+R00214
NADPH production rate Minium 32 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum -1 R02736+R01528+R00267+R00214
NADPH production rate Minium 80 R02736+R01528
Growth Condition
Strains B. megaterium MS941
Genotype The delta nprM mutant lacks a major extracellular protease (NprM) which was derived by gene replacement.
Culture medium For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution.
Carbon source Glucose
Growth rate 0.11/h
Case-specific description Metabolic flux distribution of B.megaterium MS941 strain at C-limited cultivation conditions with the glucose uptake rate of 0.11/h.
Growth Condition
Strains B. megaterium MS941
Genotype The delta nprM mutant lacks a major extracellular protease (NprM) which was derived by gene replacement.
Culture medium For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution.
Carbon source Glucose
Growth rate 0.426/h
Case-specific description Metabolic flux distribution of B.megaterium MS941 strain at C-limited cultivation conditions with the glucose uptake rate of 0.426/h.
Growth Condition
Strains B. megaterium MS941
Genotype The delta nprM mutant lacks a major extracellular protease (NprM) which was derived by gene replacement.
Culture medium For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution.
Carbon source Glucose
Growth rate 0.11/h
Case-specific description Metabolic flux distribution of B.megaterium MS941 strain at C-limited cultivation conditions with the glucose uptake rate of 0.11/h(second run).
Growth Condition
Strains B. megaterium MS941
Genotype The delta nprM mutant lacks a major extracellular protease (NprM) which was derived by gene replacement.
Culture medium For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution.
Carbon source Glucose
Growth rate 0.108/h
Case-specific description Metabolic flux distribution of B.megaterium MS941 strain at N-limited cultivation conditions with the glucose uptake rate of 0.108/h.
Growth Condition
Strains B. megaterium DSM319
Genotype Parental strain.
Culture medium For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution.
Carbon source Glucose
Growth rate 0.106/h
Case-specific description Metabolic flux distribution of B.megaterium DSM319 strain at C-limited cultivation conditions with the glucose uptake rate of 0.106/h.
Growth Condition
Strains B. megaterium WH320
Genotype WH320, a delta lacZ mutant of DSM319, was received by chemical mutagenesis using ethyl methanesulfonate.
Culture medium For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution.
Carbon source Glucose
Growth rate 0.096/h
Case-specific description Metabolic flux distribution of B.megaterium WH320 strain at C-limited cultivation conditions with the glucose uptake rate of 0.096/h.
Growth Condition
Strains B. megaterium WH323
Genotype The xylose-deficient strain WH323 was derived from WH320 by inserting the lacZ gene into the xylA locus of WH320.
Culture medium For the cultivations, a defined minimal medium was used, containing the following components (per litre):0.896 g tricine, 0.106 g MgCl2-6H2O, 0.048 g K2SO4, 0.014 g FeSO4-7H2O, 0.147 g CaCl2-2H2O, 0.02 g MnCl2-4H2O, 2.92 g NaCl,0.747 g KCl,2.0 g (NH4)2SO4, 3.52 g KH2PO4, 7.26 g Na2HPO4-2H2O, 100 uL Sigma Antifoam 204, 10 uL trace element solution.The trace element solution contains(per litre) 0.426 g (NH4)6Mo7O24-4H2O, 2.5 g H3BO3, 0.7 g CoCl2H2O, 0.25g CuSO4-5H2O, 1.6 g MnCl2-4H2O, 0.3 g ZnSO4-7H2O.The medium is adjusted to pH 7 by adding 5M KOH solution.
Carbon source Glucose
Growth rate 0.107/h
Case-specific description Metabolic flux distribution of B.megaterium WH323 strain at C-limited cultivation conditions with the glucose uptake rate of 0.107/h.
Specific Rate (mmol g-1 h-1)
glucose 1.62
CO2 4.48
acetate 0.17
Specific Rate (mmol g-1 h-1)
glucose 5.17
CO2 11.61
acetate 0.6
Specific Rate (mmol g-1 h-1)
glucose 1.58
CO2 4.52
acetate 0.15
Specific Rate (mmol g-1 h-1)
glucose 1.47
CO2 4.19
acetate 0.13
Specific Rate (mmol g-1 h-1)
glucose 1.52
CO2 4.6
acetate 0.15
Specific Rate (mmol g-1 h-1)
glucose 1.31
CO2 4
acetate 0.17
Specific Rate (mmol g-1 h-1)
glucose 1.53
CO2 3.9
acetate 0.16
Source