J Biol Chem. 2012 Aug;287(33):27959-70. 13C-flux analysis reveals NADPH-balancing transhydrogenation cycles in stationary phase of nitrogen-starving Bacillus subtilis. Ruhl M, Le Coq D, Aymerich S,et al.
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| ATP production rate Maximum | 276.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 211.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 96 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 70 | R02736+R01528 |
| ATP production rate Maximum | 1154.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 892 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 233 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 128 | R02736+R01528 |
| Growth Condition | |
| Strains | B. subtilis 168CA |
| Genotype | Wild-type,trpC2 |
| Culture medium | The nitrogen starvation medium contained per liter of deionized water: 8.5 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 0.5 g of NaCl and adjusted pH 7.The following components were added separately (per liter of final medium) 10 mL of 1 M MgSO4, 10 mL of 0.1 M CaCl2,and 20 mL of glucose 25%(w/v),followed by filter sterilization. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Relative distribution of intracellular fluxes in exponentially growing batch(glucose uptake rate of 8.2 mmol/g/h). |
| Growth Condition | |
| Strains | B. subtilis 168CA |
| Genotype | Wild-type,trpC2 |
| Culture medium | The nitrogen starvation medium contained per liter of deionized water: 8.5 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 0.5 g of NaCl and adjusted pH 7.The following components were added separately (per liter of final medium) 10 mL of 1 M MgSO4, 10 mL of 0.1 M CaCl2,and 20 mL of glucose 25%(w/v),followed by filter sterilization. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Relative distribution of intracellular fluxes in nitrogen starvation-induced resting B.subtilis cultures(glucose uptake rate of 2.0 mmol/g/h). |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.92 |
| Acetoin | 0.83 |
| citrate | 0.25 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 1.99 |
| Acetoin | 0.82 |
| citrate | 0.28 |