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J Biol Chem. 2012 Aug;287(33):27959-70. 13C-flux analysis reveals NADPH-balancing transhydrogenation cycles in stationary phase of nitrogen-starving Bacillus subtilis. Ruhl M, Le Coq D, Aymerich S,et al.



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ATP production rate Maximum 276.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 211.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 96 R02736+R01528+R00267+R00214
NADPH production rate Minium 70 R02736+R01528
ATP production rate Maximum 1154.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 892 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 233 R02736+R01528+R00267+R00214
NADPH production rate Minium 128 R02736+R01528
Growth Condition
Strains B. subtilis 168CA
Genotype Wild-type,trpC2
Culture medium The nitrogen starvation medium contained per liter of deionized water: 8.5 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 0.5 g of NaCl and adjusted pH 7.The following components were added separately (per liter of final medium) 10 mL of 1 M MgSO4, 10 mL of 0.1 M CaCl2,and 20 mL of glucose 25%(w/v),followed by filter sterilization.
Carbon source Glucose
Growth rate
Case-specific description Relative distribution of intracellular fluxes in exponentially growing batch(glucose uptake rate of 8.2 mmol/g/h).
Growth Condition
Strains B. subtilis 168CA
Genotype Wild-type,trpC2
Culture medium The nitrogen starvation medium contained per liter of deionized water: 8.5 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 0.5 g of NaCl and adjusted pH 7.The following components were added separately (per liter of final medium) 10 mL of 1 M MgSO4, 10 mL of 0.1 M CaCl2,and 20 mL of glucose 25%(w/v),followed by filter sterilization.
Carbon source Glucose
Growth rate
Case-specific description Relative distribution of intracellular fluxes in nitrogen starvation-induced resting B.subtilis cultures(glucose uptake rate of 2.0 mmol/g/h).
Specific Rate (mmol g-1 h-1)
glucose 1.92
Acetoin 0.83
citrate 0.25
Specific Rate (mmol g-1 h-1)
glucose 1.99
Acetoin 0.82
citrate 0.28
Source