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Biotechnol Bioeng. 2013 Nov;110(11):3013-23. Systems-wide analysis and engineering of metabolic pathway fluxes in bio-succinate producing Basfia succiniciproducens. Becker J, Reinefeld J, Stellmacher R, et al.



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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium N/A R02736+R01528
Growth Condition
Strains Basfia succiniciproducens
Genotype Wide type.
Culture medium For the first pre-cultivation, a complex medium containing 50g/L glucose, 5g/L yeast extract, 5g/L peptone ,1g/L NaCl, 0.2g/L MgCl2-6H2O, 0.2g/L CaCl2-2H2O, 3g/L K2HPO4, 1g/L (NH4)2SO4, and 30g/L MgCO3 was used. Second pre-cultivation and main cultivation were performed inminimal medium with 50g/Lg lucose, 1g/ NaCl, 0.2g/L MgCl2-6H2O, 0.2g/L CaCl2-2H2O, 3g/L K2HPO4, 5g/L (NH4)2SO4 and 50g/L MgCO3, 0.1g/L Ca-pantothenate, 5mgL biotin, 5mg/L cyanocobalamin, 30mg/L nicotinamide, 10mg/L pyridoxal-HCl, 6mg/L riboflavin,and 30mg/L thiamin-HCl. For tracer experiments, naturally labeled glucose was replaced by equimolar amounts of 99% [1-13C] glucose. All solutions were filter sterilized. For genetic engineering and strain maintenance, complex medium with 37g/L BHI(Becton Dickinson) was used. If required, 18g/L agar, was added to obtain solid medium.
Carbon source Glucose
Growth rate 0.3/h
Case-specific description The carbon flux distribution in the central metabolism of the wild type during growth on glucose with the mean specific glucose uptake rate of q=7.7mmol/g/h.
Specific Rate (mmol g-1 h-1)
glucose 7.7
Succinate 5.8
Formate 5.3
acetate 4.6
L-Lactic acid 0.2
Ethanol 0.8
Pyruvate 0
Source