J Biol Chem. 2010 Dec;285(50):39544-50. Metabolic flux analysis of the mixotrophic metabolisms in the green sulfur bacterium Chlorobaculum tepidum. Feng X, Tang KH, Blankenship RE, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.8 | R02736+R01528 |
| Growth Condition | |
| Strains | Chlorobaculum tepidum |
| Genotype | Wild type. |
| Culture medium | The medium composition(1 liter) was Na2EDTA-2H2O(0.015g),MgSO4-7H2O(0.22g),CaCl2-2H2O(0.08g),NaCl(0.45g),NH4Cl(0.45g),Na2S2O3-5H2O(2.6g),KH2PO4(0.57g), MOPS buffer(2.4g),trace element stock(1.2mL),vitamin B12 stock(0.08mg),NaHCO3(2.3g),and Na2SH2O(0.8g). |
| Carbon source | Acetate and NaHCO3 |
| Growth rate | 0.17/h |
| Case-specific description | Metabolic flux distribution in C.tepidum based on [2-13C] acetate culture(biomass yield is 6.4). |
| Growth Condition | |
| Strains | Chlorobaculum tepidum |
| Genotype | Wild type. |
| Culture medium | The medium composition(1 liter) was Na2EDTA-2H2O(0.015g),MgSO4-7H2O(0.22g),CaCl2-2H2O(0.08g),NaCl(0.45g),NH4Cl(0.45g),Na2S2O3-5H2O(2.6g),KH2PO4(0.57g), MOPS buffer(2.4g),trace element stock(1.2mL),vitamin B12 stock(0.08mg),NaHCO3(2.3g),and Na2SH2O(0.8g). |
| Carbon source | Pyruvate and NaHCO3 |
| Growth rate | 0.12/h |
| Case-specific description | Metabolic flux distribution in C.tepidum based on [3-13C] pyruvate culture(biomass yield is 9.2). |
| Specific Rate | (mmol g-1 h-1) |
| Acetate | 1 |
| Pyruvate | N/A |
| Specific Rate | (mmol g-1 h-1) |
| Acetate | N/A |
| Pyruvate | 0.4 |