Metab Eng. 2010 Jul;12(4):392-400. 13C metabolic flux analysis for larger scale cultivation using gas chromatography-combustion-isotope ratio mass spectrometry. Yuan Y, Yang TH, Heinzle E.
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| ATP production rate Maximum | 671.2 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 463.95 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 164.1 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 81.2 | R02736+R01528 |
| ATP production rate Maximum | 731.95 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 514.7 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 169.1 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 82.2 | R02736+R01528 |
| ATP production rate Maximum | 654.3 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 446.05 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 171.5 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 88.2 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 13032 |
| Genotype | Wild type. |
| Culture medium | cultivated in 25 ml baffled shake flasks, cultivations with low fractions of [1-13C] glucose from 0% to 10% were already described previously |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | The carbon fluxes in the central metabolism of C. glutamicum ATCC 13032 are estimated based on GC-C-IRMS method using combined labeling experiments with 0.5%, 1% and 2% [1-13C] glucose. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 13032 |
| Genotype | Wild type. |
| Culture medium | with high fractions of mixtures of [1-13C] glucose (99% isotope purity) and natural labeled glucose, 20%, 50%,80%, and 100% (w/w) [1-13C] glucose for reference |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | The carbon fluxes in the central metabolism of C.glutamicum ATCC 13032 are estimated based on GC-MS method using 99% [1-13C] glucose. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 13032 |
| Genotype | Wild type. |
| Culture medium | |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | The carbon fluxes in the central metabolism of C.glutamicum ATCC 13032 are estimated from literature. |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |