J Bacteriol. 2007 Feb;189(3):940-9. Pathway confirmation and flux analysis of central metabolic pathways in Desulfovibrio vulgaris hildenborough using gas chromatography-mass spectrometry and Fourier transform-ion cyclotron resonance mass spectrometry. Tang Y, Pingitore F, Mukhopadhyay A, et al.
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| ATP production rate Maximum | 240.25 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 240.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | -0.1 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| Growth Condition | |
| Strains | Desulfovibrio vulgaris Hildenborough(ATCC 29579) |
| Genotype | |
| Culture medium | Isotopic labeling experiments were run in triplicate using LS4D medium containing 99% L-[1-13C]lactate with a 1:10 inoculation volume. A culture in the exponential-growth phase (optical density at 600 nm, 0.4) was used as the inoculum for subcultures. To remove the effects of unlabeled lactate and glycerol from the initial stock culture, two sequential 10% subcultures into a labeled medium were performed to obtain the final culture. |
| Carbon source | Lactate. |
| Growth rate | 0.1/h |
| Case-specific description | Flux distribution in D.vulgaris Hildenborough in LS4D lactate medium. Fluxes were estimated from measurements taken at mid-log phase (30 to 40 h) and normalized by the average lactate uptake rate in log phase(13.8mmol/h/g/[dry weight]). |
| Specific Rate | (mmol g-1 h-1) |
| L-Lactic acid | 13.8 |