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Biotechnol Bioeng. 2014 Jan;111(1):202-8. IsoDesign: a software for optimizing the design of 13C-metabolic flux analysis experiments. Millard P, Sokol S, Letisse F, et al.

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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
NADPH production rate Maximum 72 R02736+R01528+R00267+R00214
NADPH production rate Minium 2 R02736+R01528
Growth Condition
Strains Escherichia coli K-12 MG1655
Culture medium The optimal label input was not a pure isotopic form but amixture of 40% U-13C-acetate, 30% 2-13C-acetate, and 30% 12C-acetate.
Carbon source Acetate
Growth rate
Case-specific description Experimental flux distribution in the central carbon metabolism of E.coli K-12 MG1655 grown on acetate ,obtained using the optimal label input identified using IsoDesign.
Specific Rate (mmol g-1 h-1)