Biotechnol Bioeng. 2014 Jan;111(1):202-8. IsoDesign: a software for optimizing the design of 13C-metabolic flux analysis experiments. Millard P, Sokol S, Letisse F, et al.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 72 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 2 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli K-12 MG1655 |
| Genotype | |
| Culture medium | The optimal label input was not a pure isotopic form but amixture of 40% U-13C-acetate, 30% 2-13C-acetate, and 30% 12C-acetate. |
| Carbon source | Acetate |
| Growth rate | |
| Case-specific description | Experimental flux distribution in the central carbon metabolism of E.coli K-12 MG1655 grown on acetate ,obtained using the optimal label input identified using IsoDesign. |
| Specific Rate | (mmol g-1 h-1) |