Biotechnol Bioeng. 2009 Apr;102(5):1377-86. Analysis of metabolic pathways and fluxes in a newly discovered thermophilic and ethanol-tolerant Geobacillus strain. Tang YJ, Sapra R, Joyner D, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Geobacillus thermoglucosidasius M10EXG |
| Genotype | |
| Culture medium | A complete minimal medium was used (Fong et al.2006) for the cell culture under defined conditions. Since singly labeled carbon substrate can well resolve the Entner-Doudoroff pathway and pentose phosphate pathway, [1-13C] D-glucose was used as the sole carbon source (Fischeret al. 2004). For anaerobic or micro-aerobic experiments, the cultures were incubated in sealed glass bottles with septum caps, and the headspace was filled with argon (anaerobic conditions) orair (micro-aerobic conditions, air liquid volume ratio 1:1). |
| Carbon source | D-Glucose |
| Growth rate | 0.31/h |
| Case-specific description | Pathways and flux distributions of glucose metabolism under aerobic conditions. |
| Growth Condition | |
| Strains | Geobacillus thermoglucosidasius M10EXG |
| Genotype | |
| Culture medium | A complete minimal medium was used (Fong et al.2006) for the cell culture under defined conditions. Since singly labeled carbon substrate can well resolve the Entner-Doudoroff pathway and pentose phosphate pathway, [1-13C] D-glucose was used as the sole carbon source (Fischeret al. 2004). For anaerobic or micro-aerobic experiments, the cultures were incubated in sealed glass bottles with septum caps, and the headspace was filled with argon (anaerobic conditions) orair (micro-aerobic conditions, air liquid volume ratio 1:1). |
| Carbon source | D-Glucose |
| Growth rate | 0.20/h |
| Case-specific description | Pathways and flux distributions of glucose metabolism under microaerobic conditions. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 5 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 5 |