Appl Environ Microbiol. 2007 Jun;73(12):3859-64. Flux analysis of central metabolic pathways in Geobacter metallireducens during reduction of soluble Fe(III)-nitrilotriacetic acid. Tang YJ, Chakraborty R, Martin HG, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 91.1 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 90.3 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.6 | R02736+R01528 |
| Growth Condition | |
| Strains | Geobacter metallireducens GS-15 |
| Genotype | |
| Culture medium | GS-15 grew in minimal medium and completely oxidized acetate as the sole carbon and energy source by reducing Fe3+ to Fe2+.All media and solutions were prepared using strict anaerobic techniques. The standard Geobacter metallireducens bicarbonate-buffered freshwater medium was used with one exception: one-tenth of the vitamin mix solution was used to minimize the isotopomer measurement “noise” introduced by unlabeled carbon from vitamins. Briefly, the medium was boiled under a N2-CO2 (80-20, vol/vol) headspace in order to remove the dissolved oxygen. |
| Carbon source | Acetate |
| Growth rate | |
| Case-specific description | Metabolic flux distribution in Geobacter metallireducens GS-15 under Fe3+ reduction conditions based on [1-13C] acetate experiments. The acetate uptake rate was 21mmol/gdw/h. |
| Growth Condition | |
| Strains | Geobacter metallireducens GS-15 |
| Genotype | |
| Culture medium | GS-15 grew in minimal medium and completely oxidized acetate as the sole carbon and energy source by reducing Fe3+ to Fe2+.All media and solutions were prepared using strict anaerobic techniques. The standard Geobacter metallireducens bicarbonate-buffered freshwater medium was used with one exception: one-tenth of the vitamin mix solution was used to minimize the isotopomer measurement “noise” introduced by unlabeled carbon from vitamins. Briefly, the medium was boiled under a N2-CO2 (80-20, vol/vol) headspace in order to remove the dissolved oxygen. |
| Carbon source | Acetate |
| Growth rate | |
| Case-specific description | Metabolic flux distribution in Geobacter metallireducens GS-15 under Fe3+ reduction conditions based on [2-13C] acetate experiments. The acetate uptake rate was 21mmol/gdw/h. |
| Specific Rate | (mmol g-1 h-1) |
| acetate | 21 |
| Specific Rate | (mmol g-1 h-1) |
| acetate | 21 |