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Appl Microbiol Biotechnol. 2013 Jul;97(14):6189-99. Metabolic flux analysis of Gluconacetobacter xylinus for bacterial cellulose production. Zhong C, Zhang GC, Liu M, et al.



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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 72.99 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 168.38 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 116.5 R02736+R01528
Growth Condition
Strains Gluconacetobacter xylinus (CGMCC no. 2955)
Genotype
Culture medium The components of the culture medium were as follows: carbon source (0.833 mol C/L), peptone (10 g/L), yeast extract (7.5 g/L), and disodium phosphate (10 g/L). Mixed carbon source medium contained the same components as the culture medium except the carbon source, which consisted of glucose, fructose, and glycerol with a ratio of 1:1:1 (w/w/w)to a final concentration of 25 g/L. G. xylinus (CGMCC no.2955) was inoculated into 100 mL of inoculum culture medium (using glucose as the carbon source) in a 500 mL flask.
Carbon source D-Glucose
Growth rate
Case-specific description Metabolic network of G.xylinus (CGMCC no. 2955) cultured with glucose as carbon source.
Growth Condition
Strains Gluconacetobacter xylinus (CGMCC no. 2955)
Genotype
Culture medium The components of the culture medium were as follows: carbon source (0.833 mol C/L), peptone (10 g/L), yeast extract (7.5 g/L), and disodium phosphate (10 g/L). Mixed carbon source medium contained the same components as the culture medium except the carbon source, which consisted of glucose, fructose, and glycerol with a ratio of 1:1:1 (w/w/w)to a final concentration of 25 g/L. G. xylinus (CGMCC no.2955) was inoculated into 100 mL of inoculum culture medium (using glucose as the carbon source) in a 500 mL flask.
Carbon source Fructose
Growth rate
Case-specific description Metabolic network of G.xylinus (CGMCC no. 2955) cultured with fructose as carbon source.
Growth Condition
Strains Gluconacetobacter xylinus (CGMCC no. 2955)
Genotype
Culture medium The components of the culture medium were as follows: carbon source (0.833 mol C/L), peptone (10 g/L), yeast extract (7.5 g/L), and disodium phosphate (10 g/L). Mixed carbon source medium contained the same components as the culture medium except the carbon source, which consisted of glucose, fructose, and glycerol with a ratio of 1:1:1 (w/w/w)to a final concentration of 25 g/L. G. xylinus (CGMCC no.2955) was inoculated into 100 mL of inoculum culture medium (using glucose as the carbon source) in a 500 mL flask.
Carbon source Glycerol
Growth rate
Case-specific description Metabolic network of G.xylinus (CGMCC no. 2955) cultured with glycerol as carbon source.
Specific Rate (mmol g-1 h-1)
Specific Rate (mmol g-1 h-1)
Specific Rate (mmol g-1 h-1)
Source