Metab Eng. 2013 Jan;15:206-17. Isotopically nonstationary 13C flux analysis of Myc-induced metabolic reprogramming in B-cells. Murphy TA, Dang CV, Young JD.
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| ATP production rate Maximum | 511.34 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 377.39 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 56.68 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 3.1 | R02736+R01528 |
| ATP production rate Maximum | 153.92 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 99.24 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 28.75 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 6.88 | R02736+R01528 |
| Growth Condition | |
| Strains | human P493-6 B-cell line |
| Genotype | The human P493-6 B-cell line expresses an EBNA2-estrogen receptor fusion protein and contains a tetracycline-repressible human MYC construct. |
| Culture medium | Medium glucose labeling was assessed by GC–MS analysis.100 mL of medium was washed with three volumes of cold acetone and centrifuged to remove protein. Samples were then evaporated to dryness at 60 Celsius under air flow. Glucose was then converted to its aldonitrile pentapropionate derivative using the same procedure described in the previous section for ribose analysis. |
| Carbon source | D-Glucose |
| Growth rate | 0.0293/h |
| Case-specific description | P493-6 B-cell flux maps determined under high Myc condition(no addition). |
| Growth Condition | |
| Strains | human P493-6 B-cell line |
| Genotype | The human P493-6 B-cell line expresses an EBNA2-estrogen receptor fusion protein and contains a tetracycline-repressible human MYC construct. |
| Culture medium | Medium glucose labeling was assessed by GC–MS analysis.100 mL of medium was washed with three volumes of cold acetone and centrifuged to remove protein. Samples were then evaporated to dryness at 60 Celsius under air flow. Glucose was then converted to its aldonitrile pentapropionate derivative using the same procedure described in the previous section for ribose analysis. |
| Carbon source | D-Glucose |
| Growth rate | 0.0176/h |
| Case-specific description | P493-6 B-cell flux maps determined under low Myc condition(Tet+BES). |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |