Biotechnol Bioeng. 2003 Oct;84(1):45-55. Quantification of central metabolic fluxes in the facultative methylotroph methylobacterium extorquens AM1 using 13C-label tracing and mass spectrometry. Van Dien SJ, Strovas T, Lidstrom ME.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Methylobacterium extorquens AM1 |
| Genotype | Wild type. |
| Culture medium | Cultures of wild-type M.extorquens AM1 were grown aerobically in liquid batch cultures using mineral salts medium containing 0.005 volume fraction (v/v) total methanol. |
| Carbon source | Methanol. |
| Growth rate | |
| Case-specific description | Metabolic fluxes in the central metabolism of wild-type M.extorquens AM1 grown in a methanol-limited chemostat on 0.001 v/v methanol at a growth rate of approximately 80% of maximum(0.11/h). |
| Growth Condition | |
| Strains | Methylobacterium extorquens AM1 phaR mutant |
| Genotype | phaR::KmR |
| Culture medium | Cultures of the phaR mutant strain were grown aerobically in liquid batch cultures using mineral salts medium containing 0.005 volume fraction (v/v) total methanol. Kanamycin was added to 10ug/mL during preculturing, but not for the experiment itself. |
| Carbon source | Methanol. |
| Growth rate | |
| Case-specific description | Metabolic fluxes in the central metabolism of an M.Extorquens AM1 phaR mutant strain grown in a methanol-limited chemostat on 0.0015 v/v methanol at a growth rate of approximately 80% of maximum(0.11/h). |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |