N Biotechnol. 2014 Jan;31(1):120-32. Metabolic flux analysis of recombinant Pichia pastoris growing on different glycerol/methanol mixtures by iterative fitting of NMR-derived (13)C-labelling data from proteinogenic amino acids. Jorda J, de Jesus SS, Peltier S, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 273.18 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.14 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 149.54 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.1 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 102.08 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.06 | R02736+R01528 |
| Growth Condition | |
| Strains | Pichia pastoris X-33/pPICZaA-ROL |
| Genotype | |
| Culture medium | Chemostat cultures were fed with a defined minimal medium containing per litre of deionized water:yeast nitrogen base 0.17 g;(NH4)2SO4, 5 g and the corresponding amount of glycerol and methanol up to 10 g, and antifoam Mazu DF7960 0.1 mL. The different compositions of glycerol and methanol, used in this study were,respectively, 80:20 (w/w), 60:40 (w/w) and 40:60 (w/w). The YNB components were sterilized separately by microfiltration before addition to the bioreactor. The medium used for starter cultures was YPD medium containing 1% (w/v) yeast extract, 2% (w/v)peptone, 2% (w/v) dextrose. |
| Carbon source | Glycerol and methanol. |
| Growth rate | |
| Case-specific description | Metabolic flux distribution in the P.pastoris recombinant strain consuming 80% glycerol 20% methanol in chemostat cultures at 0.05/h. |
| Growth Condition | |
| Strains | Pichia pastoris X-33/pPICZaA-ROL |
| Genotype | |
| Culture medium | Chemostat cultures were fed with a defined minimal medium containing per litre of deionized water:yeast nitrogen base 0.17 g;(NH4)2SO4, 5 g and the corresponding amount of glycerol and methanol up to 10 g, and antifoam Mazu DF7960 0.1 mL. The different compositions of glycerol and methanol, used in this study were,respectively, 80:20 (w/w), 60:40 (w/w) and 40:60 (w/w). The YNB components were sterilized separately by microfiltration before addition to the bioreactor. The medium used for starter cultures was YPD medium containing 1% (w/v) yeast extract, 2% (w/v)peptone, 2% (w/v) dextrose. |
| Carbon source | Glycerol and methanol. |
| Growth rate | |
| Case-specific description | Metabolic flux distribution in the P.pastoris recombinant strain consuming 60% glycerol 40% methanol in chemostat cultures at 0.05/h. |
| Growth Condition | |
| Strains | Pichia pastoris X-33/pPICZaA-ROL |
| Genotype | |
| Culture medium | Chemostat cultures were fed with a defined minimal medium containing per litre of deionized water:yeast nitrogen base 0.17 g;(NH4)2SO4, 5 g and the corresponding amount of glycerol and methanol up to 10 g, and antifoam Mazu DF7960 0.1 mL. The different compositions of glycerol and methanol, used in this study were,respectively, 80:20 (w/w), 60:40 (w/w) and 40:60 (w/w). The YNB components were sterilized separately by microfiltration before addition to the bioreactor. The medium used for starter cultures was YPD medium containing 1% (w/v) yeast extract, 2% (w/v)peptone, 2% (w/v) dextrose. |
| Carbon source | Glycerol and methanol. |
| Growth rate | |
| Case-specific description | Metabolic flux distribution in the P.pastoris recombinant strain consuming 40% glycerol 60% methanol in chemostat cultures at 0.05/h. |
| Specific Rate | (mmol g-1 h-1) |
| Glycerol | -0.92 |
| Methanol | -0.59 |
| O2 | -2.35 |
| CO2 | 1.62 |
| Specific Rate | (mmol g-1 h-1) |
| Glycerol | -0.73 |
| Methanol | -1.47 |
| O2 | -3.06 |
| CO2 | 1.95 |
| Specific Rate | (mmol g-1 h-1) |
| Glycerol | -0.55 |
| Methanol | -2.41 |
| O2 | -3.65 |
| CO2 | 2.2 |