PLoS One. 2014 Apr;9(4):e88368. Robustness and plasticity of metabolic pathway flux among uropathogenic isolates of Pseudomonas aeruginosa. Berger A, Dohnt K, Tielen P, et al.
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| ATP production rate Maximum | 773.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 594.75 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 180.8 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 109.3 | R02736+R01528 |
| ATP production rate Maximum | 970.1 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 716.35 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 206.3 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 104.8 | R02736+R01528 |
| Growth Condition | |
| Strains | Pseudomonas aeruginosa PAO1 |
| Genotype | |
| Culture medium | Starter cultures were grown in complex LB medium containing 5 g/L yeast extract, 10 g/L peptone and 10g/L NaCl. For the second and main cultures, a minimal medium developed during the study ensured balanced growth of all strains, an importantprerequisite for the13C-flux approach. The osmolality of this medium was 500 mosmol/kg, which reflects that ofhuman urine. The medium contained the following (per liter): 2.5 g glucose, 13.0 g KH2PO4-2H2O, 20.6 g K2HPO4, 0.49 g MgSO4-7H2O, 2.5 g NH4Cl, 1.41 g Na2SO4, 0.085 g CaCl2-2H2O, 1.2 mg FeSO4-7H2O, and 25 mg 3,4-dihydroxybenzoate. For 13C-flux experiments, naturally labeled glucose was replaced with 99% [1-13C] glucose. |
| Carbon source | Glucose |
| Growth rate | 0.91/h |
| Case-specific description | In vivo carbon flux distribution in the central metabolism of P.aeruginosa PAO1.Glucose uptake rate=9.5 mmol/g/h |
| Growth Condition | |
| Strains | uropathogenic Pseudomonas aeruginosa |
| Genotype | |
| Culture medium | Starter cultures were grown in complex LB medium containing 5 g/L yeast extract, 10 g/L peptone and 10g/L NaCl. For the second and main cultures, a minimal medium developed during the study ensured balanced growth of all strains, an importantprerequisite for the13C-flux approach. The osmolality of this medium was 500 mosmol/kg, which reflects that ofhuman urine. The medium contained the following (per liter): 2.5 g glucose, 13.0 g KH2PO4-2H2O, 20.6 g K2HPO4, 0.49 g MgSO4-7H2O, 2.5 g NH4Cl, 1.41 g Na2SO4, 0.085 g CaCl2-2H2O, 1.2 mg FeSO4-7H2O, and 25 mg 3,4-dihydroxybenzoate. For 13C-flux experiments, naturally labeled glucose was replaced with 99% [1-13C] glucose. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | In vivo carbon flux distribution in the central metabolism of uropathogenic P.aeruginosa isolates during growth on glucose.Flux is given as average flux of all strains and is expressed as a molar percentage of the average glucose uptake rate of all strains (8.6 mmol/g/h).(Uropathogenic P.aeruginosa isolates from patients with direct urinary tract infections included the strains MH06u, MH09u, RN12u, RN13u, MH16u, MH17u, MH26u, and MH29u.Isolates from patients with catheter-associated urinary tract infectionsincluded the strains MH15c, MH25c, MH33c, MH34c, MH36c,MH37c, MH39c, MH56c, and MH57c.) |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 9.52 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 8.6 |