J Bacteriol. 2005 Mar;187(5):1581-90. Experimental identification and quantification of glucose metabolism in seven bacterial species. Fuhrer T, Fischer E, Sauer U.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Rhodobacter sphaeroides ATH2.4.1 |
| Genotype | |
| Culture medium | The M9 minimal medium contained, per liter of deionized water,7.52 g of Na2HPO4-2H2O,3.0 g of KH2PO4, 0.5 g of NaCl,and 2.5 g of (NH4)2SO4.The following components (in indicated quantities per liter of final medium) were sterilized separately and then added: 1 mL of 0.1 M CaCl2, 1 mL of 1 M MgSO4, 0.6 mL of 100 mM FeCl3, 2 ml of vitamin solution (filter sterilized),and 10 mL of M9 trace salts solution. The vitamin solution contained (per 50 ml) 25 mg each of biotin, cyanocobalamin, niacin, calcium pantothenate, pyridoxine HCl,and thiamine HCl.For R.sphaeroides, we used a special trace salts solution that contained (per liter) 1.5 g of nitrilotriacetic acid, 3.0 g of MgSO4-7H2O, 0.5 g of MnSO4-H2O, 1.0 g of NaCl, 0.1 g of FeSO4-7H2O, 0.1 g of CoCl2-6H2O, 0.135 g of CaCl2-2H2O, 0.1 g of ZnSO4-7H2O, 0.01 g of CuSO4-5H2O, 0.01 g of H3BO3, 0.01 g of Na2MoO4-2H2O, 0.015 g of NiCl2,and 0.02 g of Na2SeO3;the pH was adjusted to 6.5 with KOH. |
| Carbon source | Glucose |
| Growth rate | 0.15/h |
| Case-specific description | In vivo carbon flux distribution in R.sphaeroides. The fluxes were normalized to the glucose uptake rate of 1.9 mmol/g/h. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 1.8 |