J Biol Chem. 2014 Jan;289(4):1960-70. Non-growing Rhodopseudomonas palustris increases the hydrogen gas yield from acetate by shifting from the glyoxylate shunt to the tricarboxylic acid cycle. McKinlay JB, Oda Y, Ruhl M, et al.
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| ATP production rate Maximum | 193.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 173.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 10 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 2 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 4 | R02736+R01528 |
| Growth Condition | |
| Strains | Rhodopseudomonas palustris CGA009 |
| Genotype | Wild type. |
| Culture medium | Strains were grown in a chemically defined medium, NFM, with N2 gas as the sole nitrogen source in sealed,anaerobic 160 ml serum vials that were stirred with stir bars or in 27 ml anaerobic tubes. |
| Carbon source | Acetate |
| Growth rate | |
| Case-specific description | Metabolic flux distributions determined by fitting simulated mass isotopomer and extracelluar flux values to measured values using 13C-FLUX2. When growing, R. palustris uses the glyoxylate shunt to conserve carbon and electrons for biosynthesis.Actual acetate uptake rates were 0.042umol/mg/DCW/h. |
| Growth Condition | |
| Strains | Rhodopseudomonas palustris CGA009 |
| Genotype | Wild type. |
| Culture medium | Strains were grown in a chemically defined medium, NFM, with N2 gas as the sole nitrogen source in sealed,anaerobic 160 ml serum vials that were stirred with stir bars or in 27 ml anaerobic tubes. |
| Carbon source | Acetate |
| Growth rate | |
| Case-specific description | Metabolic flux distributions determined by fitting simulated mass isotopomer and extracelluar flux values to measured values using 13C-FLUX2. When not growing, R. palustris oxidizes acetate via the TCA cycle. The extra reducing power generated by the TCA cycle under non-growing conditions is coupled to H2 production.Actual acetate uptake rates were 2.0 umol/mg/DCW/h. |
| Specific Rate | (mmol g-1 h-1) |
| Acetate | 0.042 |
| Specific Rate | (mmol g-1 h-1) |
| Acetate | 2 |