Microb Cell Fact. 2012 Oct;11:136. Scheffersomyces stipitis: a comparative systems biology study with the Crabtree positive yeast Saccharomyces cerevisiae. Papini M, Nookaew I, Uhlen M, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 43.17 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 39 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 165.03 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 70.22 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 178.13 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 81.54 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 168.82 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 71.12 | R02736+R01528 |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae CEN.PK113-7D |
| Genotype | Wide type. |
| Culture medium | The mineral salt medium used is composed of (per liter): (NH4)2SO4, 5 g; KH2PO4, 3 g; MgSO4-7H2O, 0.5 g; Antifoam 289, 0.05 mL;trace metals, 1 mL and vitamins, 1 mL trace metal solution. The trace metal solution consisted of (per liter): EDTA (sodium salt), 15 g; ZnSO4-7H2O, 0.45 g; MnCl2-2H2O, 1 g; CoCl2-6H2O, 0.3 g; CuSO4-5H2O,0.3 g; Na2MoO4-2H2O, 0.4 g; CaCl2-2H2O, 0.45 g; FeSO4-7H2O, 0.3 g; H3BO3, 0.1 g and KI, 0.1 g. The pH of the trace metal solution was adjusted to 4.0 with 2 MNaOH prior to heat sterilization. The vitamin solution contained (per liter): biotin, 0.05 g; p-amino benzoic acid, 0.2 g; nicotinic acid, 1 g; Ca-pantothenate, 1 g; pyridoxine-HCl, 1 g; thiamine-HCl, 1 g and myo-inositol, 25 g. The pH of the vitamin solution was adjusted to 6.5 with 2 M NaOH. |
| Carbon source | Glucose |
| Growth rate | 0.40/h |
| Case-specific description | Intracellular flux distribution of S.cerevisiae resolved during batch on glucose 5 g/L as carbon source. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae CEN.PK113-7D |
| Genotype | Wide type. |
| Culture medium | The mineral salt medium used is composed of (per liter): (NH4)2SO4, 5 g; KH2PO4, 3 g; MgSO4-7H2O, 0.5 g; Antifoam 289, 0.05 mL;trace metals, 1 mL and vitamins, 1 mL trace metal solution. The trace metal solution consisted of (per liter): EDTA (sodium salt), 15 g; ZnSO4-7H2O, 0.45 g; MnCl2-2H2O, 1 g; CoCl2-6H2O, 0.3 g; CuSO4-5H2O,0.3 g; Na2MoO4-2H2O, 0.4 g; CaCl2-2H2O, 0.45 g; FeSO4-7H2O, 0.3 g; H3BO3, 0.1 g and KI, 0.1 g. The pH of the trace metal solution was adjusted to 4.0 with 2 MNaOH prior to heat sterilization. The vitamin solution contained (per liter): biotin, 0.05 g; p-amino benzoic acid, 0.2 g; nicotinic acid, 1 g; Ca-pantothenate, 1 g; pyridoxine-HCl, 1 g; thiamine-HCl, 1 g and myo-inositol, 25 g. The pH of the vitamin solution was adjusted to 6.5 with 2 M NaOH. |
| Carbon source | Glucose |
| Growth rate | 0.10/h |
| Case-specific description | Intracellular flux distribution of S.cerevisiae resolved during chemostat cultivations on glucose 2 g/L at dilution rate 0.1/h. |
| Growth Condition | |
| Strains | Scheffersomyces stipitis CBS 6054 |
| Genotype | |
| Culture medium | The mineral salt medium used is composed of (per liter): (NH4)2SO4, 5 g; KH2PO4, 3 g; MgSO4-7H2O, 0.5 g; Antifoam 289, 0.05 mL;trace metals, 1 mL and vitamins, 1 mL trace metal solution. The trace metal solution consisted of (per liter): EDTA (sodium salt), 15 g; ZnSO4-7H2O, 0.45 g; MnCl2-2H2O, 1 g; CoCl2-6H2O, 0.3 g; CuSO4-5H2O,0.3 g; Na2MoO4-2H2O, 0.4 g; CaCl2-2H2O, 0.45 g; FeSO4-7H2O, 0.3 g; H3BO3, 0.1 g and KI, 0.1 g. The pH of the trace metal solution was adjusted to 4.0 with 2 MNaOH prior to heat sterilization. The vitamin solution contained (per liter): biotin, 0.05 g; p-amino benzoic acid, 0.2 g; nicotinic acid, 1 g; Ca-pantothenate, 1 g; pyridoxine-HCl, 1 g; thiamine-HCl, 1 g and myo-inositol, 25 g. The pH of the vitamin solution was adjusted to 6.5 with 2 M NaOH. |
| Carbon source | Glucose |
| Growth rate | 0.47/h |
| Case-specific description | Intracellular flux distribution of S.stipitis resolved during batch on glucose 5 g/L as carbon source. |
| Growth Condition | |
| Strains | Scheffersomyces stipitis CBS 6054 |
| Genotype | |
| Culture medium | The mineral salt medium used is composed of (per liter): (NH4)2SO4, 5 g; KH2PO4, 3 g; MgSO4-7H2O, 0.5 g; Antifoam 289, 0.05 mL;trace metals, 1 mL and vitamins, 1 mL trace metal solution. The trace metal solution consisted of (per liter): EDTA (sodium salt), 15 g; ZnSO4-7H2O, 0.45 g; MnCl2-2H2O, 1 g; CoCl2-6H2O, 0.3 g; CuSO4-5H2O,0.3 g; Na2MoO4-2H2O, 0.4 g; CaCl2-2H2O, 0.45 g; FeSO4-7H2O, 0.3 g; H3BO3, 0.1 g and KI, 0.1 g. The pH of the trace metal solution was adjusted to 4.0 with 2 MNaOH prior to heat sterilization. The vitamin solution contained (per liter): biotin, 0.05 g; p-amino benzoic acid, 0.2 g; nicotinic acid, 1 g; Ca-pantothenate, 1 g; pyridoxine-HCl, 1 g; thiamine-HCl, 1 g and myo-inositol, 25 g. The pH of the vitamin solution was adjusted to 6.5 with 2 M NaOH. |
| Carbon source | Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Intracellular flux distribution of S.stipitis resolved during chemostat cultivations on glucose 2 g/L at dilution rate 0.1/h. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 84.5 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 84.5 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 26.7 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 26.7 |