Appl Microbiol Biotechnol. 2013 Jun;97(11):5013-26. Metabolic fluxes in Schizosaccharomyces pombe grown on glucose and mixtures of glycerol and acetate. Klein T, Heinzle E, Schneider K.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Schizosaccharomyces pombe 972h-(ATCC 24843) |
| Genotype | Wild type. |
| Culture medium | For growth on glycerol/acetate mixtures, a minimal media was used consisting of glycerol, 9.3 g/L; sodium acetate, 4.1 g/L; NH4SO4,15.0 g/L; KH2PO4, 11.0 g/L; MgCl2, 1.0 g/L; NaCl,1.0 g/L; and CaCl2, 14.0 mg/L. Vitamins and minerals were added to the following final concentrations: calcium pantothenate, 1.0 mg/L; nicotinic acid, 10.0 mg/L; myoinositol, 10.0 mg/L; pyridoxine, 0.5 mg/L; biotin,0.01 mg/L; FeSO4, 0.7 mg/L; ZnSO4, 0.8 mg/L;MnSO4, 0.8 mg/L; boric acid, 1.0 mg/L; CoCl2, 1.0 mg/L; NaMoO4, 5.0 mg/L; potassium iodide, 2.0 mg/L;and CuSO4, 0.08 mg/L. The pH of the media was set to 5.5. For chemostat cultivations on glucose, a previously described medium was used. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Intracellular carbon flux distributions of S.pombe: cultivated in a chemostat under respirative growth conditions (D=0.1/h) on [1-13C] glucose. |
| Growth Condition | |
| Strains | Schizosaccharomyces pombe 972h-(ATCC 24843) |
| Genotype | Wild type. |
| Culture medium | For growth on glycerol/acetate mixtures, a minimal media was used consisting of glycerol, 9.3 g/L; sodium acetate, 4.1 g/L; NH4SO4,15.0 g/L; KH2PO4, 11.0 g/L; MgCl2, 1.0 g/L; NaCl,1.0 g/L; and CaCl2, 14.0 mg/L. Vitamins and minerals were added to the following final concentrations: calcium pantothenate, 1.0 mg/L; nicotinic acid, 10.0 mg/L; myoinositol, 10.0 mg/L; pyridoxine, 0.5 mg/L; biotin,0.01 mg/L; FeSO4, 0.7 mg/L; ZnSO4, 0.8 mg/L;MnSO4, 0.8 mg/L; boric acid, 1.0 mg/L; CoCl2, 1.0 mg/L; NaMoO4, 5.0 mg/L; potassium iodide, 2.0 mg/L;and CuSO4, 0.08 mg/L. The pH of the media was set to 5.5. For chemostat cultivations on glucose, a previously described medium was used. |
| Carbon source | mixture of glycerol and acetate |
| Growth rate | 0.11/h |
| Case-specific description | Intracellular carbon flux distributions of S.pombe: cultivated in a mixture of glycerol and acetate. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 1.08 |
| Acetate | 2.57 |
| O2 | 2.69 |
| CO2 | 2.75 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 0 |
| Acetate | 0.72 |
| O2 | 4.76 |
| CO2 | 4.81 |