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Appl Microbiol Biotechnol. 2013 Jun;97(11):5013-26. Metabolic fluxes in Schizosaccharomyces pombe grown on glucose and mixtures of glycerol and acetate. Klein T, Heinzle E, Schneider K.



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ATP production rate Maximum 702.79 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 466.67 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 222.23 R02736+R01528+R00267+R00214
NADPH production rate Minium 127.78 R02736+R01528
ATP production rate Maximum 182.83 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 122.02 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 260.8 R02736+R01528+R00267+R00214
NADPH production rate Minium 236.48 R02736+R01528
Growth Condition
Strains Schizosaccharomyces pombe 972h-(ATCC 24843)
Genotype Wild type.
Culture medium For growth on glycerol/acetate mixtures, a minimal media was used consisting of glycerol, 9.3 g/L; sodium acetate, 4.1 g/L; NH4SO4,15.0 g/L; KH2PO4, 11.0 g/L; MgCl2, 1.0 g/L; NaCl,1.0 g/L; and CaCl2, 14.0 mg/L. Vitamins and minerals were added to the following final concentrations: calcium pantothenate, 1.0 mg/L; nicotinic acid, 10.0 mg/L; myoinositol, 10.0 mg/L; pyridoxine, 0.5 mg/L; biotin,0.01 mg/L; FeSO4, 0.7 mg/L; ZnSO4, 0.8 mg/L;MnSO4, 0.8 mg/L; boric acid, 1.0 mg/L; CoCl2, 1.0 mg/L; NaMoO4, 5.0 mg/L; potassium iodide, 2.0 mg/L;and CuSO4, 0.08 mg/L. The pH of the media was set to 5.5. For chemostat cultivations on glucose, a previously described medium was used.
Carbon source Glucose
Growth rate
Case-specific description Intracellular carbon flux distributions of S.pombe: cultivated in a chemostat under respirative growth conditions (D=0.1/h) on [1-13C] glucose.
Growth Condition
Strains Schizosaccharomyces pombe 972h-(ATCC 24843)
Genotype Wild type.
Culture medium For growth on glycerol/acetate mixtures, a minimal media was used consisting of glycerol, 9.3 g/L; sodium acetate, 4.1 g/L; NH4SO4,15.0 g/L; KH2PO4, 11.0 g/L; MgCl2, 1.0 g/L; NaCl,1.0 g/L; and CaCl2, 14.0 mg/L. Vitamins and minerals were added to the following final concentrations: calcium pantothenate, 1.0 mg/L; nicotinic acid, 10.0 mg/L; myoinositol, 10.0 mg/L; pyridoxine, 0.5 mg/L; biotin,0.01 mg/L; FeSO4, 0.7 mg/L; ZnSO4, 0.8 mg/L;MnSO4, 0.8 mg/L; boric acid, 1.0 mg/L; CoCl2, 1.0 mg/L; NaMoO4, 5.0 mg/L; potassium iodide, 2.0 mg/L;and CuSO4, 0.08 mg/L. The pH of the media was set to 5.5. For chemostat cultivations on glucose, a previously described medium was used.
Carbon source mixture of glycerol and acetate
Growth rate 0.11/h
Case-specific description Intracellular carbon flux distributions of S.pombe: cultivated in a mixture of glycerol and acetate.
Specific Rate (mmol g-1 h-1)
Glucose 1.08
Acetate 2.57
O2 2.69
CO2 2.75
Specific Rate (mmol g-1 h-1)
Glucose 0
Acetate 0.72
O2 4.76
CO2 4.81
Source