Metab Eng. 2014 Jul;24:173-80. Metabolic network reconstruction, growth characterization and 13C-metabolic flux analysis of the extremophile Thermus thermophilus HB8. Swarup A, Lu J, DeWoody KC, et al.
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| ATP production rate Maximum | 1156.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 941.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 86 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| Growth Condition | |
| Strains | Thermus thermophilus HB8(ATCC 27634) |
| Genotype | |
| Culture medium | The optimized minimum medium contained (per liter of medium): 0.50 g K2HPO4, 0.30 g KH2PO4, 0.50 g NH4Cl, 0.50 g NaCl, 0.20 g MgCl2-6H2O, 0.04 g CaSO4-2H2O, 40 mL of 1 M Tris, 5 mL of Wolfes minerals, and 5 mL of Wolfes vitamins. The pH of the medium was 7.9. |
| Carbon source | Glucose. |
| Growth rate | 0.25/h |
| Case-specific description | Metabolic flux distributions in central metabolic pathways of T.thermophilus HB8 during exponential growth on minimum medium at 70 celsius. |
| Specific Rate | (mmol g-1 h-1) |