Mol Biosyst. 2014 Jul. Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Schatschneider S, Huber C, Neuweger H,et al.
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| ATP production rate Maximum | 1402.1 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 1122.1 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 211.8 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 99.8 | R02736+R01528 |
| Growth Condition | |
| Strains | Xanthomonas campestris pv.campestris B100 |
| Genotype | Wild type |
| Culture medium | Pre-cultures were cultivated in the modified minimal medium XMD supplemented with 0.3% glucose.XMD medium contained per liter 1g of K2HPO4-3H2O, 1g of KH2PO4, 0.6g of KNO3, 0.25g of MgSO4-7H2O, 0.1g of CaCl2-2H2O, and 0.2mg of FeCl3. For the labeling experiment, Xcc cells were grown in XMD minimal medium with 0.3% [1-13C]glucose. |
| Carbon source | Glucose |
| Growth rate | 0.05/h |
| Case-specific description | In vivocarbon flux distribution of X.campestris pv.campestris B100 as determined by GC-MS based metabolic flux analysis. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 2 |
| pentasaccharide | 0.198 |