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Mol Biosyst. 2014 Jul. Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Schatschneider S, Huber C, Neuweger H,et al.

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ATP production rate Maximum 1402.1 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
ATP production rate Minium 1122.1 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
NADPH production rate Maximum 211.8 R02736+R01528+R00267+R00214
NADPH production rate Minium 99.8 R02736+R01528
Growth Condition
Strains Xanthomonas campestris pv.campestris B100
Genotype Wild type
Culture medium Pre-cultures were cultivated in the modified minimal medium XMD supplemented with 0.3% glucose.XMD medium contained per liter 1g of K2HPO4-3H2O, 1g of KH2PO4, 0.6g of KNO3, 0.25g of MgSO4-7H2O, 0.1g of CaCl2-2H2O, and 0.2mg of FeCl3. For the labeling experiment, Xcc cells were grown in XMD minimal medium with 0.3% [1-13C]glucose.
Carbon source Glucose
Growth rate 0.05/h
Case-specific description In vivocarbon flux distribution of X.campestris pv.campestris B100 as determined by GC-MS based metabolic flux analysis.
Specific Rate (mmol g-1 h-1)
Glucose 2
pentasaccharide 0.198