Mol Biosyst. 2014 Jul. Metabolic flux pattern of glucose utilization by Xanthomonas campestris pv. campestris: prevalent role of the Entner-Doudoroff pathway and minor fluxes through the pentose phosphate pathway and glycolysis. Schatschneider S, Huber C, Neuweger H,et al.
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|ATP production rate Maximum||1402.1||-R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
|ATP production rate Minium||1122.1||-R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
|NADPH production rate Maximum||211.8||R02736+R01528+R00267+R00214|
|NADPH production rate Minium||99.8||R02736+R01528|
|Strains||Xanthomonas campestris pv.campestris B100|
|Culture medium||Pre-cultures were cultivated in the modified minimal medium XMD supplemented with 0.3% glucose.XMD medium contained per liter 1g of K2HPO4-3H2O, 1g of KH2PO4, 0.6g of KNO3, 0.25g of MgSO4-7H2O, 0.1g of CaCl2-2H2O, and 0.2mg of FeCl3. For the labeling experiment, Xcc cells were grown in XMD minimal medium with 0.3% [1-13C]glucose.|
|Case-specific description||In vivocarbon flux distribution of X.campestris pv.campestris B100 as determined by GC-MS based metabolic flux analysis.|
|Specific Rate||(mmol g-1 h-1)|