Arch Microbiol. 1999 May-Jun;171(6):371-85. Metabolic state of Zymomonas mobilis in glucose-, fructose-, and xylose-fed continuous cultures as analysed by 13C- and 31P-NMR spectroscopy. De Graaf AA, Striegel K, Wittig RM, et al.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 26 | R02736+R01528 |
| Growth Condition | |
| Strains | Z. mobilis CP4 (ATCC 31821) |
| Genotype | |
| Culture medium | Medium I: 70 g D-glucose (mixture of 80% unlabelled glucoseand 20% [2-13C]glucose), 0.87 g NH4Cl, 0.082 g MgSO4-7H2O, 0.18 g KH2PO4,0.125 g trisodium citrate, 0.0021 g FeSO4-7H2O, 1 mg Ca-pan-thotenate and 1 mg biotin. Medium II: 70 g D-fructose (mixture of 80% unlabelled fructose and 20% [2-13C]fructose), 1.6 gNH4Cl, 1.0 g MgSO4-7H2O, 3.5 g KH2PO4, 0.21 g trisodium citrate, 0.010 g FeSO4-7H2O, 1 mg Ca-panthotenate and 1 mg biotin. Medium III: 70 g D-xylose (mixture of 81% unlabelled xylose and 19% [1-13C]xylose), 1.98 g (NH4)2SO4, 1.0 g MgSO4-7H2O,3.48 g KH2PO4, 0.21 g trisodium citrate, 0.010 g FeSO4-7H2O and 10 ml of a vitamin stock solution containing (per litre) 1.65 mg biotin, 150 mg choline, 0.05 mg cyanocobalamin, 0.5 mg folic acid, 0.7 mg inositol, 299 mg nicotinic acid, 382 mg p-aminoben zoic acid, 137 mg panthotenic acid, 21.6 mg pyridoxine, 58.3 mg riboflavin and 265 mg thiamine. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | TKT transketolase [(a) and (b)merely denote the two different reactions shown], and TAL transaldolase. XI, XK and TAL are absent from wild-type Z. mobilis, |
| Growth Condition | |
| Strains | Z. mobilis CP4 (ATCC 31821) |
| Genotype | |
| Culture medium | Medium I: 70 g D-glucose (mixture of 80% unlabelled glucoseand 20% [2-13C]glucose), 0.87 g NH4Cl, 0.082 g MgSO4-7H2O, 0.18 g KH2PO4,0.125 g trisodium citrate, 0.0021 g FeSO4-7H2O, 1 mg Ca-pan-thotenate and 1 mg biotin. Medium II: 70 g D-fructose (mixture of 80% unlabelled fructose and 20% [2-13C]fructose), 1.6 gNH4Cl, 1.0 g MgSO4-7H2O, 3.5 g KH2PO4, 0.21 g trisodium citrate, 0.010 g FeSO4-7H2O, 1 mg Ca-panthotenate and 1 mg biotin. Medium III: 70 g D-xylose (mixture of 81% unlabelled xylose and 19% [1-13C]xylose), 1.98 g (NH4)2SO4, 1.0 g MgSO4-7H2O,3.48 g KH2PO4, 0.21 g trisodium citrate, 0.010 g FeSO4-7H2O and 10 ml of a vitamin stock solution containing (per litre) 1.65 mg biotin, 150 mg choline, 0.05 mg cyanocobalamin, 0.5 mg folic acid, 0.7 mg inositol, 299 mg nicotinic acid, 382 mg p-aminoben zoic acid, 137 mg panthotenic acid, 21.6 mg pyridoxine, 58.3 mg riboflavin and 265 mg thiamine. |
| Carbon source | Fructose |
| Growth rate | |
| Case-specific description | TKT transketolase [(a) and (b)merely denote the two different reactions shown], and TAL transaldolase. XI, XK and TAL are absent from wild-type Z. mobilis, |
| Growth Condition | |
| Strains | Z. mobilis CP4/pZY228/pZY557tal |
| Genotype | |
| Culture medium | Medium I: 70 g D-glucose (mixture of 80% unlabelled glucoseand 20% [2-13C]glucose), 0.87 g NH4Cl, 0.082 g MgSO4-7H2O, 0.18 g KH2PO4,0.125 g trisodium citrate, 0.0021 g FeSO4-7H2O, 1 mg Ca-pan-thotenate and 1 mg biotin. Medium II: 70 g D-fructose (mixture of 80% unlabelled fructose and 20% [2-13C]fructose), 1.6 gNH4Cl, 1.0 g MgSO4-7H2O, 3.5 g KH2PO4, 0.21 g trisodium citrate, 0.010 g FeSO4-7H2O, 1 mg Ca-panthotenate and 1 mg biotin. Medium III: 70 g D-xylose (mixture of 81% unlabelled xylose and 19% [1-13C]xylose), 1.98 g (NH4)2SO4, 1.0 g MgSO4-7H2O,3.48 g KH2PO4, 0.21 g trisodium citrate, 0.010 g FeSO4-7H2O and 10 ml of a vitamin stock solution containing (per litre) 1.65 mg biotin, 150 mg choline, 0.05 mg cyanocobalamin, 0.5 mg folic acid, 0.7 mg inositol, 299 mg nicotinic acid, 382 mg p-aminoben zoic acid, 137 mg panthotenic acid, 21.6 mg pyridoxine, 58.3 mg riboflavin and 265 mg thiamine. |
| Carbon source | Xylose |
| Growth rate | |
| Case-specific description | the heterologous enzymes (in addition to heterologous TKT) are present in the xylose-degrading strain Z. mobilis CP4/pZY228/pZY557tal used in this study |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 31.5 |
| Fructose | N/A |
| Xylose | N/A |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | N/A |
| Fructose | 28.14 |
| Xylose | N/A |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | N/A |
| Fructose | N/A |
| Xylose | 6.48 |