Metab Eng. 2007 Mar;9(2):177-92. Determining Actinobacillus succinogenes metabolic pathways and fluxes by NMR and GC-MS analyses of 13C-labeled metabolic product isotopomers. McKinlay JB, Shachar-Hill Y, Zeikus JG, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 10.24 | R02736+R01528 |
| Growth Condition | |
| Strains | Actinobacillus succinogenes 130Z (ATCC 55618) |
| Genotype | Wild type. |
| Culture medium | AM3 medium is a chemically defined medium containing Cys, Met, and Glu, which are required for A.Succinogenes growth. |
| Carbon source | Glucose |
| Growth rate | 0.28/h |
| Case-specific description | Anoxic AM3 contained 150 mM NaHCO3 and 50 mM glucose. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 7.9 |
| Succinate | 5.8 |
| Formate | 3.5 |
| Acetate | 4.4 |
| Fumarate | 0.2 |
| Ethanol | 1.2 |

