Metab Eng. 2000 Jan;2(1):34-41. Construction and characterization of an oxalic acid nonproducing strain of Aspergillus niger. Pedersen H, Christensen B, Hjort C, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 514 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 116 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 382.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 134 | R02736+R01528 |
| Growth Condition | |
| Strains | Aspergillus niger BO-1 |
| Genotype | Wild type,producing oxalic acid as a by-product which causes problems with downstream processing of industrial enzymes. |
| Culture medium | The Aspergillus strains were grown in a defined medium containing: 15g/L glucose monohydrate, 7.3g/L (NH4)2SO4, 1.5g/L KH2PO4, 1.0g/L MgSO4-7H2O, 1.0g/L NaCl, 0.1g/L CaCl2-2H2O, 0.5ml/L sb2121, 7.2mg/L ZnSO4-7H2O, 1.3mg/L CuSO4-5H2O, 0.3mg/L NiCl2-6H2O, and 6.9mg/L FeSO4-7H2O. |
| Carbon source | Glucose |
| Growth rate | 0.19/h |
| Case-specific description | Metabolic fluxes in the central metabolism of A.niger BO-1 during batch cultivation (BAN26). |
| Growth Condition | |
| Strains | A.niger oah disruptant 4-1 |
| Genotype | The oah gene encoding oxaloacetate hydrolase (EC 3.7.1.1) was disrupted in a glucoamylase-producing strain of A. niger and the resulting strain was incapable of producing oxalic acid. |
| Culture medium | The Aspergillus strains were grown in a defined medium containing: 15g/L glucose monohydrate, 7.3g/L (NH4)2SO4, 1.5g/L KH2PO4, 1.0g/L MgSO4-7H2O, 1.0g/L NaCl, 0.1g/L CaCl2-2H2O, 0.5ml/L sb2121, 7.2mg/L ZnSO4-7H2O, 1.3mg/L CuSO4-5H2O, 0.3mg/L NiCl2-6H2O, and 6.9mg/L FeSO4-7H2O. |
| Carbon source | Glucose |
| Growth rate | 0.18/h |
| Case-specific description | Metabolic fluxes in the central metabolism of 4-1 during batch cultivation (BAN41). |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 10.29 |
| citrate | 4.96 |
| Oxalic | 4.29 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 9.58 |
| citrate | 4.035 |
| Oxalic | 0 |