J Biotechnol. 2007 Dec;132(4):385-94. Effect of different carbon sources on central metabolic fluxes and the recombinant production of a hydrolase from Thermobifida fusca in Bacillus megaterium. Furch T, Wittmann C, Wang W, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 159 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 78 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 191 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 114 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | B.megaterium WH323 |
| Genotype | B.megaterium WH323(lacZ-,xylA::lacZ) was transformed with the plasmid pYYBm9 carrying the gene for a heterologous hydrolase from T.fusca (referred to as TFH) under the control of the xylose inducible PxylA promoter. |
| Culture medium | For all cultivations a defined minimal medium was used,containing the following components: 5 mM tricine, 520 uM MgCl2-6H2O, 276 uM K2SO4,50 uM FeSO4-7H2O, 1 mM CaCl2-2H2O, 100 uM MnCl2-4H2O, 50 mM NaCl, 10 mM KCl, 0.2%(w/v) (NH4)2SO4, 26 mM KH2PO4, 40 mM Na2HPO4-2H2O, 100 uL Antifoam 204 and 10 uL trace element solution per liter medium.The trace element solution contained 300 uM (NH4)6Mo7O24-4H2O, 40 mM H3BO3, 3 mM CoCl2-6H2O, 1 mM CuSO4-5H2O, 8 mM MnCl2-4H2O and 1 mM ZnSO4-7H2O.The medium was adjusted to pH 7 by adding 5 M KOH solution. |
| Carbon source | Glucose |
| Growth rate | 0.107/h |
| Case-specific description | Metabolic flux distribution of strain WH323 carrying TFH encoding pYYBm9 under cultivation conditions of non-induced gene expression with glucose as the sole carbon source. |
| Growth Condition | |
| Strains | B.megaterium WH323 |
| Genotype | B.megaterium WH323(lacZ-,xylA::lacZ) was transformed with the plasmid pYYBm9 carrying the gene for a heterologous hydrolase from T.fusca (referred to as TFH) under the control of the xylose inducible PxylA promoter. |
| Culture medium | For all cultivations a defined minimal medium was used,containing the following components: 5 mM tricine, 520 uM MgCl2-6H2O, 276 uM K2SO4,50 uM FeSO4-7H2O, 1 mM CaCl2-2H2O, 100 uM MnCl2-4H2O, 50 mM NaCl, 10 mM KCl, 0.2%(w/v) (NH4)2SO4, 26 mM KH2PO4, 40 mM Na2HPO4-2H2O, 100 uL Antifoam 204 and 10 uL trace element solution per liter medium.The trace element solution contained 300 uM (NH4)6Mo7O24-4H2O, 40 mM H3BO3, 3 mM CoCl2-6H2O, 1 mM CuSO4-5H2O, 8 mM MnCl2-4H2O and 1 mM ZnSO4-7H2O.The medium was adjusted to pH 7 by adding 5 M KOH solution. |
| Carbon source | Glucose |
| Growth rate | 0.107/h |
| Case-specific description | Metabolic flux distribution of strain WH323 carrying TFH encoding pYYBm9 under cultivation conditions of induced gene expression with glucose as the sole carbon source. |
| Growth Condition | |
| Strains | B.megaterium WH323 |
| Genotype | B.megaterium WH323(lacZ-,xylA::lacZ) was transformed with the plasmid pYYBm9 carrying the gene for a heterologous hydrolase from T.fusca (referred to as TFH) under the control of the xylose inducible PxylA promoter. |
| Culture medium | For all cultivations a defined minimal medium was used,containing the following components: 5 mM tricine, 520 uM MgCl2-6H2O, 276 uM K2SO4,50 uM FeSO4-7H2O, 1 mM CaCl2-2H2O, 100 uM MnCl2-4H2O, 50 mM NaCl, 10 mM KCl, 0.2%(w/v) (NH4)2SO4, 26 mM KH2PO4, 40 mM Na2HPO4-2H2O, 100 uL Antifoam 204 and 10 uL trace element solution per liter medium.The trace element solution contained 300 uM (NH4)6Mo7O24-4H2O, 40 mM H3BO3, 3 mM CoCl2-6H2O, 1 mM CuSO4-5H2O, 8 mM MnCl2-4H2O and 1 mM ZnSO4-7H2O.The medium was adjusted to pH 7 by adding 5 M KOH solution. |
| Carbon source | Pyruvate |
| Growth rate | 0.103/h |
| Case-specific description | Metabolic flux distribution of strain WH323 carrying TFH encoding pYYBm9 under cultivation conditions of non-induced gene expression with pyruvate as the sole carbon source. |
| Growth Condition | |
| Strains | B.megaterium WH323 |
| Genotype | B.megaterium WH323(lacZ-,xylA::lacZ) was transformed with the plasmid pYYBm9 carrying the gene for a heterologous hydrolase from T.fusca (referred to as TFH) under the control of the xylose inducible PxylA promoter. |
| Culture medium | For all cultivations a defined minimal medium was used,containing the following components: 5 mM tricine, 520 uM MgCl2-6H2O, 276 uM K2SO4,50 uM FeSO4-7H2O, 1 mM CaCl2-2H2O, 100 uM MnCl2-4H2O, 50 mM NaCl, 10 mM KCl, 0.2%(w/v) (NH4)2SO4, 26 mM KH2PO4, 40 mM Na2HPO4-2H2O, 100 uL Antifoam 204 and 10 uL trace element solution per liter medium.The trace element solution contained 300 uM (NH4)6Mo7O24-4H2O, 40 mM H3BO3, 3 mM CoCl2-6H2O, 1 mM CuSO4-5H2O, 8 mM MnCl2-4H2O and 1 mM ZnSO4-7H2O.The medium was adjusted to pH 7 by adding 5 M KOH solution. |
| Carbon source | Pyruvate |
| Growth rate | 0.103/h |
| Case-specific description | Metabolic flux distribution of strain WH323 carrying TFH encoding pYYBm9 under cultivation conditions of induced gene expression with pyruvate as the sole carbon source. |
| Specific Rate | (mmol g-1 h-1) |
| CO2 | 3.9 |
| Acetate | 0.16 |
| glucose | 1.53 |
| pyruvate | N/A |
| Specific Rate | (mmol g-1 h-1) |
| CO2 | 4.09 |
| Acetate | 0.18 |
| glucose | 1.5 |
| pyruvate | N/A |
| Specific Rate | (mmol g-1 h-1) |
| CO2 | 5.64 |
| Acetate | 0.16 |
| glucose | N/A |
| pyruvate | 3.4 |
| Specific Rate | (mmol g-1 h-1) |
| CO2 | 5.56 |
| Acetate | 0.23 |
| glucose | N/A |
| pyruvate | 3.45 |