Biotechnol Bioeng. 2012 Mar;109(3):763-71. Collisional fragmentation of central carbon metabolites in LC-MS/MS increases precision of 13 C metabolic flux analysis. Ruhl M, Rupp B, Noh K, et al.
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| ATP production rate Maximum | 252.2 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 190.2 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 94.4 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 69.6 | R02736+R01528 |
| ATP production rate Maximum | 275.15 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 209.4 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 96.9 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 70.6 | R02736+R01528 |
| Growth Condition | |
| Strains | B. subtilis BSB168trp+ |
| Genotype | Wild type. |
| Culture medium | The M9 minimal medium consisted per liter of deionized water: 8.5 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 1 g of NH4Cl, 0.5 g of NaCl,and was adjusted to pH 7 before filter sterilization.The following components were filter sterilized separately and then added (per liter of final medium): 1 mL of 1 M MgSO4, 1 mL of 0.1 M CaCl2, 1 mL 0.05 M FeCl3 containing 0.1 M citric acid, 20 mL of glucose 25%(w/v), 1 mL of 0.245 M tryptophan,and 10 mL of a trace element solution containing (per liter) 170 mg of ZnCl2, 100mg MnCl2-4H2O, 60 mg of CoCl2-6H2O, 60 mg Na2MoO4-2H2O,and 43 mg CuCl2-2H2O. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Intracellular flux distribution of B.subtilis wild-type during exponential growth on 20% (w/w) [U-13C] and 80% [1-13C] glucose derived from 13C-patterns of solely intact metabolic intermediates.The relative flux values are normalized to the glucose uptake rate of 8.2 mmol/g/h. |
| Growth Condition | |
| Strains | B. subtilis BSB168trp+ |
| Genotype | Wild type. |
| Culture medium | The M9 minimal medium consisted per liter of deionized water: 8.5 g of Na2HPO4-2H2O, 3.0 g of KH2PO4, 1 g of NH4Cl, 0.5 g of NaCl,and was adjusted to pH 7 before filter sterilization.The following components were filter sterilized separately and then added (per liter of final medium): 1 mL of 1 M MgSO4, 1 mL of 0.1 M CaCl2, 1 mL 0.05 M FeCl3 containing 0.1 M citric acid, 20 mL of glucose 25%(w/v), 1 mL of 0.245 M tryptophan,and 10 mL of a trace element solution containing (per liter) 170 mg of ZnCl2, 100mg MnCl2-4H2O, 60 mg of CoCl2-6H2O, 60 mg Na2MoO4-2H2O,and 43 mg CuCl2-2H2O. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Intracellular flux distribution of B.subtilis wild-type during exponential growth on 20% (w/w) [U-13C] and 80% [1-13C] glucose derived from intact and fragmented carbon backbones of metabolic intermediates.The relative flux values are normalized to the glucose uptake rate of 8.2 mmol/g/h. |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |