Login   |   Regist

Metab Eng. 2006 Sep;8(5):432-46. Respirometric 13C flux analysis--Part II: in vivo flux estimation of lysine-producing Corynebacterium glutamicum. Hoon Yang T, Wittmann C, Heinzle E.

Click on the "GO TO" button to display the flux map.

Font-size: Color: Global color
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 133.8 R02736+R01528
Growth Condition
Strains a mutant of C.glutamicum ATCC 13032
Genotype It is characterized by a feedback-resistantaspartokinasedue to a single base pair exchange oflysC beta gene, which leads to an amino acid exchange in the beta-subunit of the aspartokinase.
Culture medium The calibration medium was composed of 8 stock solutions. In all, 1L medium contained: (A) 0.055g CaCl2-2H2O, 0.2g MgSO4-7H2O, 1g NaCl in 579.5ml deionized water; (B)2g K2HPO4, 0.25g KH2PO4 in 10ml deionized water; (C)16g K2HPO4, 2g KH2PO4 in 280ml deionized water and adjusted to pH 6.5 with HCl; (D) 5g (NH4)2SO4 in 100ml deionized water; (E) 0.5mg biotin, 1mg thiamine-HCl in 20ml deionized water; (F) 5ml of 2g/LFeSO4-7H2O (20mg FeSO4-7H2O in 10ml deionized water, adjusted to pH 1.0 with HCl); (G) 5ml of 100*trace elements and (H) 30mg 3,4-dihydroxybenzoic acid in 0.5ml deionized water. The solution (G)was prepared by adding 50ml/L 4M NaOH. All solutions were sterilized by filtration. The pH of the calibration medium was 6.55 and its ionic strength 0.431M at 30 Celsius.The salting out coefficient of the calibration medium was 0.044 for O2 and 0.031 for CO2.
Carbon source Glucose
Growth rate 0.403/h
Case-specific description Relative fluxes from the multiple 13C labeling experiments with CO2 labeling measurements estimated by Monte Carlo method.
Specific Rate (mmol g-1 h-1)