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Anal Biochem. 2004 Feb;325(2):308-16. High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints. Fischer E, Zamboni N, Sauer U.



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ATP production rate Maximum 487 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 387 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 88 R02736+R01528+R00267+R00214
NADPH production rate Minium 48 R02736+R01528
ATP production rate Maximum 504 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 394 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 104 R02736+R01528+R00267+R00214
NADPH production rate Minium 60 R02736+R01528
ATP production rate Maximum 437.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 357.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 86 R02736+R01528+R00267+R00214
NADPH production rate Minium 54 R02736+R01528
Growth Condition
Strains Escherichia coli MG1655
Genotype
Culture medium Escherichia coli MG1655 was grown in a 2-liter bioreactor (LH-Inceltech) in M9 minimal medium supple-mented with 2g/liter glucose as the sole carbon source.
Carbon source Glucose
Growth rate 0.82/h
Case-specific description Metabolic net fluxes estimated with 13C-constrained flux balancing from 20/80 [U-13C]glucose/[1-13C]glucose
Growth Condition
Strains Escherichia coli MG1655
Genotype
Culture medium Escherichia coli MG1655 was grown in a 2-liter bioreactor (LH-Inceltech) in M9 minimal medium supple-mented with 2g/liter glucose as the sole carbon source.
Carbon source Glucose
Growth rate 0.82/h
Case-specific description Metabolic net fluxes estimated with comprehensive isotopomer balancing from 20/80 [U-13C]glucose/[1-13C]glucose
Growth Condition
Strains Escherichia coli MG1655
Genotype
Culture medium Escherichia coli MG1655 was grown in a 2-liter bioreactor (LH-Inceltech) in M9 minimal medium supple-mented with 2g/liter glucose as the sole carbon source.
Carbon source Glucose
Growth rate 0.65/h
Case-specific description Metabolic net fluxes refer to 13C-constrained flux balancing of a 20/80 [U-13C]glucose/[1-13C]glucose shake flask culture. Fluxes are normalized to the glucose uptake rate.
Specific Rate (mmol g-1 h-1)
glucose 11
CO2 18.6
O2 18.2
acetate 6.4
Specific Rate (mmol g-1 h-1)
glucose 7.1
CO2 N/A
O2 N/A
acetate 6.6
Specific Rate (mmol g-1 h-1)
glucose 8
CO2 N/A
O2 N/A
acetate 6.4
Source