Anal Biochem. 2004 Feb;325(2):308-16. High-throughput metabolic flux analysis based on gas chromatography-mass spectrometry derived 13C constraints. Fischer E, Zamboni N, Sauer U.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | 487 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 387 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 88 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 48 | R02736+R01528 |
| ATP production rate Maximum | 504 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 394 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 104 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 60 | R02736+R01528 |
| ATP production rate Maximum | 437.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 357.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 86 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 54 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli MG1655 |
| Genotype | |
| Culture medium | Escherichia coli MG1655 was grown in a 2-liter bioreactor (LH-Inceltech) in M9 minimal medium supple-mented with 2g/liter glucose as the sole carbon source. |
| Carbon source | Glucose |
| Growth rate | 0.82/h |
| Case-specific description | Metabolic net fluxes estimated with 13C-constrained flux balancing from 20/80 [U-13C]glucose/[1-13C]glucose |
| Growth Condition | |
| Strains | Escherichia coli MG1655 |
| Genotype | |
| Culture medium | Escherichia coli MG1655 was grown in a 2-liter bioreactor (LH-Inceltech) in M9 minimal medium supple-mented with 2g/liter glucose as the sole carbon source. |
| Carbon source | Glucose |
| Growth rate | 0.82/h |
| Case-specific description | Metabolic net fluxes estimated with comprehensive isotopomer balancing from 20/80 [U-13C]glucose/[1-13C]glucose |
| Growth Condition | |
| Strains | Escherichia coli MG1655 |
| Genotype | |
| Culture medium | Escherichia coli MG1655 was grown in a 2-liter bioreactor (LH-Inceltech) in M9 minimal medium supple-mented with 2g/liter glucose as the sole carbon source. |
| Carbon source | Glucose |
| Growth rate | 0.65/h |
| Case-specific description | Metabolic net fluxes refer to 13C-constrained flux balancing of a 20/80 [U-13C]glucose/[1-13C]glucose shake flask culture. Fluxes are normalized to the glucose uptake rate. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 11 |
| CO2 | 18.6 |
| O2 | 18.2 |
| acetate | 6.4 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 7.1 |
| CO2 | N/A |
| O2 | N/A |
| acetate | 6.6 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 8 |
| CO2 | N/A |
| O2 | N/A |
| acetate | 6.4 |