J Biosci Bioeng. 2011 Dec;112(6):616-23. Metabolite channeling and compartmentation in the human cell line AGE1.HN determined by 13C labeling experiments and 13C metabolic flux analysis. Niklas J, Sandig V, Heinzle E.
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|ATP production rate Maximum||N/A||-R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
|ATP production rate Minium||N/A||-R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
|NADPH production rate Maximum||62.2||R02736+R01528+R00267+R00214|
|NADPH production rate Minium||6.2||R02736+R01528|
|Genotype||The cell line AGE1.HN was developed from primary cells from a human brain tissue sample which were immortalized with an expression plasmid containing the adenoviral E1 A and B genes of human adenovirus type 5 driven by the human pGK and the endogenous E1B promoter, respectively.|
|Culture medium||The applied tracers were [1,2–13C] glucose (99%), [U–13C] glucose (99%), [U–13C] glutamine(99%), [U–13C3] alanine (98%) and [1–13C] lactate (20% W/W in water). The glucose and glutamine tracer experiments were carried out as follows. The cells were cultured in baffled shake flasks at 37 Celsius on a shaker enclosed in a 5% CO2 supplied, humidified (80%) incubator.|
|Case-specific description||Metabolic flux map for AGE1.HN during the first 3 days of batch cultivation.|
|Specific Rate||(mmol g-1 h-1)|