J Biosci Bioeng. 2011 Dec;112(6):616-23. Metabolite channeling and compartmentation in the human cell line AGE1.HN determined by 13C labeling experiments and 13C metabolic flux analysis. Niklas J, Sandig V, Heinzle E.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 62.2 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 6.2 | R02736+R01528 |
| Growth Condition | |
| Strains | AGE1.HN |
| Genotype | The cell line AGE1.HN was developed from primary cells from a human brain tissue sample which were immortalized with an expression plasmid containing the adenoviral E1 A and B genes of human adenovirus type 5 driven by the human pGK and the endogenous E1B promoter, respectively. |
| Culture medium | The applied tracers were [1,2–13C] glucose (99%), [U–13C] glucose (99%), [U–13C] glutamine(99%), [U–13C3] alanine (98%) and [1–13C] lactate (20% W/W in water). The glucose and glutamine tracer experiments were carried out as follows. The cells were cultured in baffled shake flasks at 37 Celsius on a shaker enclosed in a 5% CO2 supplied, humidified (80%) incubator. |
| Carbon source | D-Glucose |
| Growth rate | |
| Case-specific description | Metabolic flux map for AGE1.HN during the first 3 days of batch cultivation. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | -0.145 |
| Lactate | 0.337 |
| Pyruvate | -0.037 |