Microb Cell Fact. 2012 May;11:57. Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose: methanol mixtures. Jorda J, Jouhten P, Camara E, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 510 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 66 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 290 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 114 | R02736+R01528 |
| Growth Condition | |
| Strains | Pichia pastoris X-33 |
| Genotype | harbouring pGAPA delta as mock plasmid |
| Culture medium | Two duplicate aerobic, carbon-limited continuous cultures for each of the three strains were carried out in a 3 L vessel bioreactor (Applikon Biotechnology) that was controlled at 25 Celsius . The working volume was kept at 1 L by means of an overflow system. The pH was controlled at 5.0 with 1 M NH3. P. pastoris cells were fed with a minimal medium containing 50 g/L of a glucose:methanol mixture (80% glucose/20% methanol, w/w) for five bioreactor volume changes until reaching a metabolic steady state, as indicated by a constant cell density in the bioreactor and constant O2 and CO2 concentrations in the exhaust gas. |
| Carbon source | Glucose. |
| Growth rate | |
| Case-specific description | Metabolic flux distributions in the P.pastoris during growth in glucose and glucose:methanol chemostat cultures at about 0.1/h. |
| Growth Condition | |
| Strains | Pichia pastoris X-33 |
| Genotype | harbouring pGAPA delta as mock plasmid |
| Culture medium | Two duplicate aerobic, carbon-limited continuous cultures for each of the three strains were carried out in a 3 L vessel bioreactor (Applikon Biotechnology) that was controlled at 25 Celsius . The working volume was kept at 1 L by means of an overflow system. The pH was controlled at 5.0 with 1 M NH3. P. pastoris cells were fed with a minimal medium containing 50 g/L of a glucose:methanol mixture (80% glucose/20% methanol, w/w) for five bioreactor volume changes until reaching a metabolic steady state, as indicated by a constant cell density in the bioreactor and constant O2 and CO2 concentrations in the exhaust gas. |
| Carbon source | Glucose and methanol. |
| Growth rate | |
| Case-specific description | Metabolic flux distributions in the P.pastoris during growth in glucose and glucose:methanol chemostat cultures at about 0.1/h. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | -0.76 |
| Methanol | 0 |
| O2 | -2.95 |
| CO2 | 2.31 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | -0.76 |
| Methanol | -1.15 |
| O2 | -2.95 |
| CO2 | 2.31 |