J Biotechnol. 2009 Aug;143(2):124-9. Metabolic flux analysis of a phenol producing mutant of Pseudomonas putida S12: verification and complementation of hypotheses derived from transcriptomics. Wierckx N, Ruijssenaars HJ, de Winde JH, et al.
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| ATP production rate Maximum | 1018.62 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 688.12 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 135.58 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 3.38 | R02736+R01528 |
| ATP production rate Maximum | 1162.98 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 848.16 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 148.15 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 22.22 | R02736+R01528 |
| Growth Condition | |
| Strains | Pseudomonas putida S12JNNmcs(t) |
| Genotype | |
| Culture medium | Cultivations of P.putida S12JNNmcs(t) was performed at 30 Celsius in 500ml Erlenmeyer flasks (no baffles) containing 50ml minimal medium (Hartmans et al., 1989) with 10 mg/l gentamicin and 0.1mM salicylate as an inducer for the heterologous nagR/PnagAa promoter controlling the expression of TPL (tyrosine phenol lyase). |
| Carbon source | D-Glucose |
| Growth rate | 0.47/h |
| Case-specific description | Metabolic fluxes in P.putida S12JNNmcs(t). |
| Growth Condition | |
| Strains | Pseudomonas putida S12TPL3 |
| Genotype | |
| Culture medium | Cultivations of P.putida S12TPL3 was performed at 30 Celsius in 500ml Erlenmeyer flasks (no baffles) containing 50ml minimal medium (Hartmans et al., 1989) with 10 mg/l gentamicin and 0.1mM salicylate as an inducer for the heterologous nagR/PnagAa promoter controlling the expression of TPL (tyrosine phenol lyase). |
| Carbon source | D-Glucose |
| Growth rate | 0.42/h |
| Case-specific description | Metabolic fluxes in P.putida S12TPL3. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 5.6 |
| Phenol | 0 |
| 4-Hydroxyphenylpyruvate | 0 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 6.14 |
| Phenol | 0.18 |
| 4-Hydroxyphenylpyruvate | 0.43 |