Biotechnol Bioeng. 2001 Sep;76(2):144-56. Metabolic flux analysis with a comprehensive isotopomer model in Bacillus subtilis. Dauner M, Bailey JE, Sauer U.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Bacillus subtilis |
| Genotype | The riboflavin-producing strain RB50::[PRF69]n with an amplified recombinant rib operon. |
| Culture medium | The labelling Experiments are based on several glucose-limited chemostat experiments,which were also used for 13C-labeling experiments in physiological steady state.During these labeling experiments, the feed medium was replaced by an identical medium containing 10% uniformly [U-13C6] glucose and 90% glucose labeled at natural abundance. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Flux distribution in central carbon metabolism of B.subtilisin glucose-limited chemostat culture at low dilution rate(0.11/h). |
| Growth Condition | |
| Strains | Bacillus subtilis |
| Genotype | The riboflavin-producing strain RB50::[PRF69]n with an amplified recombinant rib operon. |
| Culture medium | The labelling Experiments are based on several glucose-limited chemostat experiments,which were also used for 13C-labeling experiments in physiological steady state.During these labeling experiments, the feed medium was replaced by an identical medium containing 10% uniformly [U-13C6] glucose and 90% glucose labeled at natural abundance. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Flux distribution in central carbon metabolism of B.subtilisin glucose-limited chemostat culture at high dilution rate(0.44/h). |
| Specific Rate | (mmol g-1 h-1) |
| riboflavin | 0.02 |
| glucose | 1.98 |
| acetate | 0.01 |
| citrate | 0.03 |
| Phosphoenolpyruvate | 0.01 |
| pyruvate | 0.01 |
| formate | 0.03 |
| succinate | 0 |
| CO2 | 6.69 |
| diacetyl | 0.09 |
| O2 | 6.71 |
| Specific Rate | (mmol g-1 h-1) |
| riboflavin | 0.03 |
| glucose | 7.05 |
| acetate | 0.09 |
| citrate | 0.06 |
| Phosphoenolpyruvate | 0.01 |
| pyruvate | 0.01 |
| formate | 0.04 |
| succinate | 0.01 |
| CO2 | 19.75 |
| diacetyl | 0.17 |
| O2 | 19.71 |