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Appl Environ Microbiol. 2004 Oct;70(10):5905-11. Oxygen- and glucose-dependent regulation of central carbon metabolism in Pichia anomala. Fredlund E, Blank LM, Schnurer J, et al.



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ATP production rate Maximum 332 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 217 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 106 R02736+R01528+R00267+R00214
NADPH production rate Minium 60 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium -98 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 20 R02736+R01528
Growth Condition
Strains Pichia anomala NRRL-Y-366-8
Genotype
Culture medium Defined media were used for all cultivations and glucose (20 g/liter) was used as the sole carbon source. The inocula were precultivated for 20 to 30 h at 25 Celsius, 150 rpm, in YNB medium with the same carbon source as that for the subsequent batch cultivation and added to the fermentor vessel to an optical density at 600 nm (OD600) of 0.1 to 0.15. All growth experiments were repeated at least three times.All experiments were performed in a Belach fermentor with a 1-liter working volume at 25 Celsius. The pH was automatically kept constant at 5.0 by titration with 2 M KOH. The dissolved oxygen tension was measured with an autoclavable O2sensor.In aerobic cultivations the oxygen saturation was kept at 50%5% by regulating the stirring velocity between 100 and 900 rpm at an aeration rate of 1liter of air min In the oxygen shift experiments, the culture was flushed with nitrogen until the dissolved oxygen tension reached 0%, which was achieved within 1 min.
Carbon source D-Glucose
Growth rate 0.22/h
Case-specific description Metabolite flux distribution in P.anomala of an aerobic culture.
Growth Condition
Strains Pichia anomala NRRL-Y-366-8
Genotype
Culture medium Defined media were used for all cultivations and glucose (20 g/liter) was used as the sole carbon source. The inocula were precultivated for 20 to 30 h at 25 Celsius, 150 rpm, in YNB medium with the same carbon source as that for the subsequent batch cultivation and added to the fermentor vessel to an optical density at 600 nm (OD600) of 0.1 to 0.15. All growth experiments were repeated at least three times.All experiments were performed in a Belach fermentor with a 1-liter working volume at 25 Celsius. The pH was automatically kept constant at 5.0 by titration with 2 M KOH. The dissolved oxygen tension was measured with an autoclavable O2sensor.In aerobic cultivations the oxygen saturation was kept at 50%5% by regulating the stirring velocity between 100 and 900 rpm at an aeration rate of 1liter of air min In the oxygen shift experiments, the culture was flushed with nitrogen until the dissolved oxygen tension reached 0%, which was achieved within 1 min.
Carbon source D-Glucose
Growth rate 0.056/h
Case-specific description Metabolite flux distribution in P.anomala of an oxygen-limited culture.
Specific Rate (mmol g-1 h-1)
Glucose 2.1
Ethanol 0.14
Glycerol 0.12
Acetate 0.22
Specific Rate (mmol g-1 h-1)
Glucose 4.6
Ethanol 6.2
Glycerol 0.61
Acetate 0
Source