Appl Environ Microbiol. 2005 Dec;71(12):8587-96. Amplified expression of fructose ,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources. Becker J, Klopprogge C, Zelder O, et al.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 418.4 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 93.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 583.7 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 112.4 | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum ATCC 13032 lysCfbr |
| Genotype | exchange T311I in the lysC gene |
| Culture medium | Complex medium containing 5g/L glucose, 5g/L yeast extract,10g/L tryptone,5g/L NaCl,and 18g/L agar was used for agar plates. |
| Carbon source | Glucose |
| Growth rate | 0.39/h |
| Case-specific description | In vivo carbon flux distribution in the central metabolism of glucose-grown Corynebacterium glutamicum ATCC 13032 lysCfbr during lysine production. All fluxes are expressed as a molar percentage of the mean specific glucose uptake rate (4.9 mmol /g/h), which was set to 100%. |
| Growth Condition | |
| Strains | Corynebacterium glutamicumATCC 13032 lysCfbr PEFTUfbp |
| Genotype | lysC T311I replacement of the natural promoter of the fbp gene (NCgI2268) with the promoter of the tuf gene |
| Culture medium | Complex medium containing 5g/L glucose, 5g/L yeast extract,10g/L tryptone,5g/L NaCl,and 18g/L agar was used for agar plates. |
| Carbon source | Glucose |
| Growth rate | 0.31/h |
| Case-specific description | In vivo carbon flux distribution in the central metabolism of glucose-grown Corynebacterium glutamicum ATCC 13032 lysCfbr PEFTUfbp during lysine production. All fluxes are expressed as a molar percentage of the mean specific glucose uptake rate (4.8 mmol/g/h), which was set to 100%. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.9 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.8 |