Metab Eng. 2013 Nov;20:49-55. COMPLETE-MFA:complementary parallel labeling experiments technique for metabolic flux analysis. Leighty RW, Antoniewicz MR.
Click on the "GO TO" button to display the flux map.
|ATP production rate Maximum||423.65||-R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
|ATP production rate Minium||383.65||-R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
|NADPH production rate Maximum||68||R02736+R01528+R00267+R00214|
|NADPH production rate Minium||52||R02736+R01528|
|Strains||Escherichia coli K-12 MG1655|
|Culture medium||Defined medium (glucose-free M9 medium) was used for all experiments.Then glucose tracers,[1-13C],[2-13C],[3-13C],[4-13C],[5-13C],and [6-13C]glucose were added,and the initial glucose concentration in each culture was 2.55 g/L.|
|Case-specific description||Wild-type E.coli was grown aerobically in six parallel batch cultures with [1-13C],[2-13C],[3-13C],[4-13C],[5-13C],and [6-13C] glucose tracers in mini-bioreactors.|
|Specific Rate||(mmol g-1 h-1)|