Metab Eng. 2013 Nov;20:49-55. COMPLETE-MFA:complementary parallel labeling experiments technique for metabolic flux analysis. Leighty RW, Antoniewicz MR.
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| ATP production rate Maximum | 423.65 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 383.65 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 68 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 52 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli K-12 MG1655 |
| Genotype | Wild type. |
| Culture medium | Defined medium (glucose-free M9 medium) was used for all experiments.Then glucose tracers,[1-13C],[2-13C],[3-13C],[4-13C],[5-13C],and [6-13C]glucose were added,and the initial glucose concentration in each culture was 2.55 g/L. |
| Carbon source | Glucose |
| Growth rate | 0.68/h |
| Case-specific description | Wild-type E.coli was grown aerobically in six parallel batch cultures with [1-13C],[2-13C],[3-13C],[4-13C],[5-13C],and [6-13C] glucose tracers in mini-bioreactors. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 100 |
| avetate | 75 |