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Metab Eng. 2013 Nov;20:49-55. COMPLETE-MFA:complementary parallel labeling experiments technique for metabolic flux analysis. Leighty RW, Antoniewicz MR.

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ATP production rate Maximum 423.65 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
ATP production rate Minium 383.65 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
NADPH production rate Maximum 68 R02736+R01528+R00267+R00214
NADPH production rate Minium 52 R02736+R01528
Growth Condition
Strains Escherichia coli K-12 MG1655
Genotype Wild type.
Culture medium Defined medium (glucose-free M9 medium) was used for all experiments.Then glucose tracers,[1-13C],[2-13C],[3-13C],[4-13C],[5-13C],and [6-13C]glucose were added,and the initial glucose concentration in each culture was 2.55 g/L.
Carbon source Glucose
Growth rate 0.68/h
Case-specific description Wild-type E.coli was grown aerobically in six parallel batch cultures with [1-13C],[2-13C],[3-13C],[4-13C],[5-13C],and [6-13C] glucose tracers in mini-bioreactors.
Specific Rate (mmol g-1 h-1)
glucose 100
avetate 75