Metab Eng. 2005 Sep-Nov;7(5-6):437-44. Comparative metabolic network analysis of two xylose fermenting recombinant Saccharomyces cerevisiae strains. Grotkjaer T, Christakopoulos P, Nielsen J, et al.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | -69.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 98.2 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | -85.35 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 128.6 | R02736+R01528 |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae CPB.CR4 |
| Genotype | |
| Culture medium | Initial batch cultivation with 2.5 g/Lglucose and 13g/L xylose was first performed, and then a medium with the same composition as the batch cultivation medium was continuously fed to the bioreactor at a dilution rate of 0.058/h. When steady state was achieved after three residence times, the feeding medium was switched to a medium containing 2.5g glucose (a mixture of 1.65g naturally labelled glucose and 0.85g [1-13C] glucose) and 13g xylose per litre until a new isotopic steady state was achieved after another three residence times (Grotkjar et al., 2004). |
| Carbon source | D-Glucose |
| Growth rate | |
| Case-specific description | Calculated net fluxes in the central metabolism of the xylose fermenting strain CPB.CR4. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae TMB3001 |
| Genotype | |
| Culture medium | Initial batch cultivation with 2.5 g/Lglucose and 13g/L xylose was first performed, and then a medium with the same composition as the batch cultivation medium was continuously fed to the bioreactor at a dilution rate of 0.058/h. When steady state was achieved after three residence times, the feeding medium was switched to a medium containing 2.5g glucose (a mixture of 1.65g naturally labelled glucose and 0.85g [1-13C] glucose) and 13g xylose per litre until a new isotopic steady state was achieved after another three residence times (Grotkjar et al., 2004). |
| Carbon source | D-Glucose |
| Growth rate | |
| Case-specific description | Calculated net fluxes in the central metabolism of S.cerevisiae for TMB3001. |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |