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Appl Environ Microbiol. 2004 Dec;70(12):7277-87. Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source. Wittmann C, Kiefer P, Zelder O.

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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
ATP production rate Minium 1643.9 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 222.8 R02736+R01528
Growth Condition
Strains Corynebacterium glutamicum ATCC 21526
Culture medium Precultures were grown in complex medium containing 5g/L of sucrose.For agar plates,the complex medium was additionally amended with 12g/L of agar.For production of cells as aninoculum for the tracer experiments,a minimal medium with 1mg/L calcium panthotenate HCl was used.
Carbon source Sucrose
Growth rate
Case-specific description In vivo carbon flux distribution in the central metabolism of C.glutamicum ATCC 21526 during lysine production on sucrose. All fluxes are expressed as molar percentages of the mean specific sucrose uptake rate (0.8 mmol/g/h).
Specific Rate (mmol g-1 h-1)
Sucrose 0.8