Nat Protoc. 2009;4(6):878-92. (13)C-based metabolic flux analysis. Zamboni N, Fendt SM, Ruhl M, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 100 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 81.8 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 207.7 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 84.6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 66.7 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 28.6 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli MG1655 |
| Genotype | Wild type |
| Culture medium | M9 medium |
| Carbon source | Glucose |
| Growth rate | 0.12/h |
| Case-specific description | 13CFLUX was used to fit the same physiological and 13C data with the reference model,fitting residuum=132. |
| Growth Condition | |
| Strains | Escherichia coli MG1655 |
| Genotype | Wild type |
| Culture medium | M9 medium |
| Carbon source | Glucose |
| Growth rate | 0.12/h |
| Case-specific description | 13CFLUX was used to fit the same physiological and 13C data with an amended model lacking the glyoxylate shunt,fitting residuum=251. |
| Growth Condition | |
| Strains | Escherichia coli MG1655 |
| Genotype | Wild type |
| Culture medium | M9 medium |
| Carbon source | Glucose |
| Growth rate | 0.12/h |
| Case-specific description | 13CFLUX was used to fit the same physiological and 13C data with a complete model with addition of the methylglyoxylate shunt in parallel to lower glycolysis ,fitting residuum=119.7. |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |