Microb Cell Fact. 2005 Nov;4:30. Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis. Frick O, Wittmann C.
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| ATP production rate Maximum | 647.4 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 446.65 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 189.7 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 109.4 | R02736+R01528 |
| ATP production rate Maximum | 557.8 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 385.55 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 128.1 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 59.2 | R02736+R01528 |
| ATP production rate Maximum | 199.55 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 90.05 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | 74 | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 30.2 | R02736+R01528 |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae ATCC 32167 |
| Genotype | |
| Culture medium | The mineral medium used for cultivation contained 2 g/L glucose, 0.5 g/L (NH4)2HPO4, 1.0 g/L (NH4)2SO4, 0.05 g/L MgSO4-7 H2O, 0.025 g/L citric acid, 0.5 g/L KCl, 0.03,g/L CaCl2-2H2O, 3 mg/L FeCl3-6 H2O, 2.1 mg/L MnSO4-H2O, 1.8 mg/L ZnSO4-7H2O, 0.5 mg/L CuSO4-5H2O, 60.3 mg/L myo-inositol, 30 mg/L Ca-pan thotenate, 6 mg/L thiamin-HCl, 1.5 mg/L pyridoxine-HCl, 0.03 mg/L biotin, and 50 mmol/L phosphate buffer (pH 6.2). |
| Carbon source | Glucose |
| Growth rate | 0.15/h |
| Case-specific description | Intracellular carbon flux distribution of S.cerevisiae cultivated in chemostat on [1-13C] glucose under aerobic glucose-limited condition,corresponding to purely oxidative growth(u=0.15/h, qglc = 1.56mmol/g/h). |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae ATCC 32167 |
| Genotype | |
| Culture medium | The mineral medium used for cultivation contained 2 g/L glucose, 0.5 g/L (NH4)2HPO4, 1.0 g/L (NH4)2SO4, 0.05 g/L MgSO4-7 H2O, 0.025 g/L citric acid, 0.5 g/L KCl, 0.03,g/L CaCl2-2H2O, 3 mg/L FeCl3-6 H2O, 2.1 mg/L MnSO4-H2O, 1.8 mg/L ZnSO4-7H2O, 0.5 mg/L CuSO4-5H2O, 60.3 mg/L myo-inositol, 30 mg/L Ca-pan thotenate, 6 mg/L thiamin-HCl, 1.5 mg/L pyridoxine-HCl, 0.03 mg/L biotin, and 50 mmol/L phosphate buffer (pH 6.2). |
| Carbon source | Glucose |
| Growth rate | 0.30/h |
| Case-specific description | Intracellular carbon flux distribution of S.cerevisiae cultivated in chemostat on [1-13C] glucose under aerobic glucose-limited condition,corresponding to respiro-fermentative growth(u=0.30/h, qglc = 4.90mmol/g/h). |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae ATCC 32167 |
| Genotype | |
| Culture medium | The mineral medium used for cultivation contained 2 g/L glucose, 0.5 g/L (NH4)2HPO4, 1.0 g/L (NH4)2SO4, 0.05 g/L MgSO4-7 H2O, 0.025 g/L citric acid, 0.5 g/L KCl, 0.03,g/L CaCl2-2H2O, 3 mg/L FeCl3-6 H2O, 2.1 mg/L MnSO4-H2O, 1.8 mg/L ZnSO4-7H2O, 0.5 mg/L CuSO4-5H2O, 60.3 mg/L myo-inositol, 30 mg/L Ca-pan thotenate, 6 mg/L thiamin-HCl, 1.5 mg/L pyridoxine-HCl, 0.03 mg/L biotin, and 50 mmol/L phosphate buffer (pH 6.2). |
| Carbon source | Glucose |
| Growth rate | 0.4/h |
| Case-specific description | Intracellular carbon flux distribution of S.cerevisiae cultivated in chemostat on [1-13C] glucose under aerobic glucose-limited condition,corresponding to mainly fermentative growth(u=0.40/h, qglc = 8.23mmol/g/h). |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 1.56 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 4.9 |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 8.23 |