Biotechnol Prog. 2000 Mar-Apr;16(2):169-75. 13C NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid formation in Bacillus subtilis. Phalakornkule C, Fry B, Zhu T, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Bacillus subtilis |
| Genotype | Wild type;tryptophan auxotroph. |
| Culture medium | A 1L portion of media contained 1g of NH4Cl, 0.04g of tryptophan, 1.0g of KH2PO4, 2.72g of K2HPO4, 0.284g of Na2SO4, 0.17g of NaNO3, 0.15g of KCl, 0.049g of MgCl2-6H2O, 2.16mg of FeCl3-6H2O, 0.015g of MnCl2-4H2O, and 0.022g of CaCl2-6H2O. The concentrations of glucose and citrate were 2 and 0.32 g/L,respectively. During the steady-state condition, the carbon sources were switched from natural abundance glucose and citrate to [1-13C]D-glucose and unlabeled citrate. |
| Carbon source | Glucose and citrate |
| Growth rate | 0.4/h |
| Case-specific description | Metabolite trafficking patterns that are predicted by linear programming to confer high carbon yield and agree to within 10% with the observed yield. Flux values correspond to basis growth rate of 0.4/h. The maximized yield solutions also correspond to minimized pyruvate kinase flux. |
| Growth Condition | |
| Strains | Bacillus subtilis |
| Genotype | Wild type;tryptophan auxotroph. |
| Culture medium | A 1L portion of media contained 1g of NH4Cl, 0.04g of tryptophan, 1.0g of KH2PO4, 2.72g of K2HPO4, 0.284g of Na2SO4, 0.17g of NaNO3, 0.15g of KCl, 0.049g of MgCl2-6H2O, 2.16mg of FeCl3-6H2O, 0.015g of MnCl2-4H2O, and 0.022g of CaCl2-6H2O. The concentrations of glucose and citrate were 2 and 0.32 g/L,respectively. During the steady-state condition, the carbon sources were switched from natural abundance glucose and citrate to [1-13C]D-glucose and unlabeled citrate. |
| Carbon source | Glucose and citrate |
| Growth rate | 0.4/h |
| Case-specific description | Metabolite trafficking patterns that are predicted by linear programming to confer high carbon yield and agree to within 10% with the observed yield. Flux values correspond to basis growth rate of 0.4/h. The maximized yield solutions also correspond to minimized hexose monophosphate pathway flux. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 17.92 |
| citrate | 0 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 7.61 |
| citrate | 7.13 |