Eur J Biochem. 1998 May;254(1):96-102. Carbon-flux distribution in the central metabolic pathways of Corynebacterium glutamicum during growth on fructose. Dominguez H, Rollin C, Guyonvarch A, et al.
Click on the "GO TO" button to display the flux map.
Font-size:
Color:
Global color
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Xylitol-resistant mutants of C.glutamicum ATCC 17965 |
| Genotype | |
| Culture medium | The growth medium was that previously described " Dominguez, H., Nezondet, C., Lindley, N. D. & Cocaign, M. (1993)Modified carbon flux during oxygen limited growth of Corynebacterium glutamicum and the consequences for amino acid overproduction, Biotechnol. Letts. 15, 4492454." except as regarding the carbon substrate which was either glucose or fructose added to obtain initial sugar concentrations of 83 mM (500 mM carbon equivalent |
| Carbon source | Fructose. |
| Growth rate | 0.48/h |
| Case-specific description | Proposed modification of the flux distribution through the tricarboxylic acid cycle to facilitate an adequate supply of NADPH for anabolic requirements during growth of C.glutamicum on fructose. |
| Growth Condition | |
| Strains | Xylitol-resistant mutants of C.glutamicum ATCC 17965 |
| Genotype | |
| Culture medium | The growth medium was that previously described " Dominguez, H., Nezondet, C., Lindley, N. D. & Cocaign, M. (1993)Modified carbon flux during oxygen limited growth of Corynebacterium glutamicum and the consequences for amino acid overproduction, Biotechnol. Letts. 15, 4492454." except as regarding the carbon substrate which was either glucose or fructose added to obtain initial sugar concentrations of 83 mM (500 mM carbon equivalent |
| Carbon source | Glucose. |
| Growth rate | 0.59/h |
| Case-specific description | The flux distribution of C.glutamicum grown on glucose. |
| Specific Rate | (mmol g-1 h-1) |
| Fructose | 4.4 |
| glucose | N/A |
| CO2 | 8.1 |
| dihydroxyacetone | 0.29 |
| lactate | 0.21 |
| Specific Rate | (mmol g-1 h-1) |
| Fructose | N/A |
| glucose | 4.7 |
| CO2 | 6.2 |
| dihydroxyacetone | N/A |
| lactate | N/A |