Biotechnol Prog. 2004 May-Jun;20(3):706-14. Serial 13C-based flux analysis of an L-phenylalanine-producing E.coli strain using the sensor reactor. Wahl A, El Massaoudi M, Schipper D, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 99.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 6 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 1183.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 108 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 1269 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 78 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | 1620.5 | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 12 | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli 4pF81 |
| Genotype | E.coli LJ110 delta(pheA tyrA aroF) pJF119EH aroF pheAfbr aroB aroL |
| Culture medium | For shaking flask cultivations,the following medium was used: 0.3 g/L MgSO4-7H2O, 0.015 g/L CaCl2-2H2O, 3.0 g/L KH2PO4, 12 g/L K2HPO4, 0.1 g/L NaCl, 5.0 g/L (NH4)2SO4, 0.075 g/L Fe(SO4)2-7H2O, 1.0 g/L Na-citrate,1.5 mL/L trace element solution, 0.0075 g/L vitamin B1 (thiamine HCl), 0.08 g/L tyrosine, 5.0 g/L glucose-H2O, 0.1 g/L ampicillin. The trace element solution consisted of: 2.0 g/L Al2(SO4)3-18H2O, 0.75 g/L CoSO4-7H2O, 2.5 g/L CuSO4-5H2O, 0.5 g/L H3BO4, 24 g/L MnSO4, 3.0 g/L Na2MoO4-2H2O, 2.5 g/L NiSO4-6H2O, 15 g/L ZnSO4-7H2O. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Metabolic network and carbon flux distributions, based on 13C labeling experiments with the Sensor reactor system,during the L-Phe production phase under tyrosine-limited conditions. The optimal flux distribution for L-Phe production (Opt) was calculated for nongrowing cells. |
| Growth Condition | |
| Strains | Escherichia coli 4pF81 |
| Genotype | E.coli LJ110 delta(pheA tyrA aroF) pJF119EH aroF pheAfbr aroB aroL |
| Culture medium | For shaking flask cultivations,the following medium was used: 0.3 g/L MgSO4-7H2O, 0.015 g/L CaCl2-2H2O, 3.0 g/L KH2PO4, 12 g/L K2HPO4, 0.1 g/L NaCl, 5.0 g/L (NH4)2SO4, 0.075 g/L Fe(SO4)2-7H2O, 1.0 g/L Na-citrate,1.5 mL/L trace element solution, 0.0075 g/L vitamin B1 (thiamine HCl), 0.08 g/L tyrosine, 5.0 g/L glucose-H2O, 0.1 g/L ampicillin. The trace element solution consisted of: 2.0 g/L Al2(SO4)3-18H2O, 0.75 g/L CoSO4-7H2O, 2.5 g/L CuSO4-5H2O, 0.5 g/L H3BO4, 24 g/L MnSO4, 3.0 g/L Na2MoO4-2H2O, 2.5 g/L NiSO4-6H2O, 15 g/L ZnSO4-7H2O. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Monitored are the periods 14-16.75h. |
| Growth Condition | |
| Strains | Escherichia coli 4pF81 |
| Genotype | E.coli LJ110 delta(pheA tyrA aroF) pJF119EH aroF pheAfbr aroB aroL |
| Culture medium | For shaking flask cultivations,the following medium was used: 0.3 g/L MgSO4-7H2O, 0.015 g/L CaCl2-2H2O, 3.0 g/L KH2PO4, 12 g/L K2HPO4, 0.1 g/L NaCl, 5.0 g/L (NH4)2SO4, 0.075 g/L Fe(SO4)2-7H2O, 1.0 g/L Na-citrate,1.5 mL/L trace element solution, 0.0075 g/L vitamin B1 (thiamine HCl), 0.08 g/L tyrosine, 5.0 g/L glucose-H2O, 0.1 g/L ampicillin. The trace element solution consisted of: 2.0 g/L Al2(SO4)3-18H2O, 0.75 g/L CoSO4-7H2O, 2.5 g/L CuSO4-5H2O, 0.5 g/L H3BO4, 24 g/L MnSO4, 3.0 g/L Na2MoO4-2H2O, 2.5 g/L NiSO4-6H2O, 15 g/L ZnSO4-7H2O. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Monitored are the periods 17.2-20h. |
| Growth Condition | |
| Strains | Escherichia coli 4pF81 |
| Genotype | E.coli LJ110 delta(pheA tyrA aroF) pJF119EH aroF pheAfbr aroB aroL |
| Culture medium | For shaking flask cultivations,the following medium was used: 0.3 g/L MgSO4-7H2O, 0.015 g/L CaCl2-2H2O, 3.0 g/L KH2PO4, 12 g/L K2HPO4, 0.1 g/L NaCl, 5.0 g/L (NH4)2SO4, 0.075 g/L Fe(SO4)2-7H2O, 1.0 g/L Na-citrate,1.5 mL/L trace element solution, 0.0075 g/L vitamin B1 (thiamine HCl), 0.08 g/L tyrosine, 5.0 g/L glucose-H2O, 0.1 g/L ampicillin. The trace element solution consisted of: 2.0 g/L Al2(SO4)3-18H2O, 0.75 g/L CoSO4-7H2O, 2.5 g/L CuSO4-5H2O, 0.5 g/L H3BO4, 24 g/L MnSO4, 3.0 g/L Na2MoO4-2H2O, 2.5 g/L NiSO4-6H2O, 15 g/L ZnSO4-7H2O. |
| Carbon source | Glucose |
| Growth rate | |
| Case-specific description | Monitored are the periods 20.5-23.3h. |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |