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Biotechnol Prog. 2004 May-Jun;20(3):706-14. Serial 13C-based flux analysis of an L-phenylalanine-producing E.coli strain using the sensor reactor. Wahl A, El Massaoudi M, Schipper D, et al.



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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 99.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 6 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 1183.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 108 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 1269 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 78 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 1620.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 12 R02736+R01528
Growth Condition
Strains Escherichia coli 4pF81
Genotype E.coli LJ110 delta(pheA tyrA aroF) pJF119EH aroF pheAfbr aroB aroL
Culture medium For shaking flask cultivations,the following medium was used: 0.3 g/L MgSO4-7H2O, 0.015 g/L CaCl2-2H2O, 3.0 g/L KH2PO4, 12 g/L K2HPO4, 0.1 g/L NaCl, 5.0 g/L (NH4)2SO4, 0.075 g/L Fe(SO4)2-7H2O, 1.0 g/L Na-citrate,1.5 mL/L trace element solution, 0.0075 g/L vitamin B1 (thiamine HCl), 0.08 g/L tyrosine, 5.0 g/L glucose-H2O, 0.1 g/L ampicillin. The trace element solution consisted of: 2.0 g/L Al2(SO4)3-18H2O, 0.75 g/L CoSO4-7H2O, 2.5 g/L CuSO4-5H2O, 0.5 g/L H3BO4, 24 g/L MnSO4, 3.0 g/L Na2MoO4-2H2O, 2.5 g/L NiSO4-6H2O, 15 g/L ZnSO4-7H2O.
Carbon source Glucose
Growth rate
Case-specific description Metabolic network and carbon flux distributions, based on 13C labeling experiments with the Sensor reactor system,during the L-Phe production phase under tyrosine-limited conditions. The optimal flux distribution for L-Phe production (Opt) was calculated for nongrowing cells.
Growth Condition
Strains Escherichia coli 4pF81
Genotype E.coli LJ110 delta(pheA tyrA aroF) pJF119EH aroF pheAfbr aroB aroL
Culture medium For shaking flask cultivations,the following medium was used: 0.3 g/L MgSO4-7H2O, 0.015 g/L CaCl2-2H2O, 3.0 g/L KH2PO4, 12 g/L K2HPO4, 0.1 g/L NaCl, 5.0 g/L (NH4)2SO4, 0.075 g/L Fe(SO4)2-7H2O, 1.0 g/L Na-citrate,1.5 mL/L trace element solution, 0.0075 g/L vitamin B1 (thiamine HCl), 0.08 g/L tyrosine, 5.0 g/L glucose-H2O, 0.1 g/L ampicillin. The trace element solution consisted of: 2.0 g/L Al2(SO4)3-18H2O, 0.75 g/L CoSO4-7H2O, 2.5 g/L CuSO4-5H2O, 0.5 g/L H3BO4, 24 g/L MnSO4, 3.0 g/L Na2MoO4-2H2O, 2.5 g/L NiSO4-6H2O, 15 g/L ZnSO4-7H2O.
Carbon source Glucose
Growth rate
Case-specific description Monitored are the periods 14-16.75h.
Growth Condition
Strains Escherichia coli 4pF81
Genotype E.coli LJ110 delta(pheA tyrA aroF) pJF119EH aroF pheAfbr aroB aroL
Culture medium For shaking flask cultivations,the following medium was used: 0.3 g/L MgSO4-7H2O, 0.015 g/L CaCl2-2H2O, 3.0 g/L KH2PO4, 12 g/L K2HPO4, 0.1 g/L NaCl, 5.0 g/L (NH4)2SO4, 0.075 g/L Fe(SO4)2-7H2O, 1.0 g/L Na-citrate,1.5 mL/L trace element solution, 0.0075 g/L vitamin B1 (thiamine HCl), 0.08 g/L tyrosine, 5.0 g/L glucose-H2O, 0.1 g/L ampicillin. The trace element solution consisted of: 2.0 g/L Al2(SO4)3-18H2O, 0.75 g/L CoSO4-7H2O, 2.5 g/L CuSO4-5H2O, 0.5 g/L H3BO4, 24 g/L MnSO4, 3.0 g/L Na2MoO4-2H2O, 2.5 g/L NiSO4-6H2O, 15 g/L ZnSO4-7H2O.
Carbon source Glucose
Growth rate
Case-specific description Monitored are the periods 17.2-20h.
Growth Condition
Strains Escherichia coli 4pF81
Genotype E.coli LJ110 delta(pheA tyrA aroF) pJF119EH aroF pheAfbr aroB aroL
Culture medium For shaking flask cultivations,the following medium was used: 0.3 g/L MgSO4-7H2O, 0.015 g/L CaCl2-2H2O, 3.0 g/L KH2PO4, 12 g/L K2HPO4, 0.1 g/L NaCl, 5.0 g/L (NH4)2SO4, 0.075 g/L Fe(SO4)2-7H2O, 1.0 g/L Na-citrate,1.5 mL/L trace element solution, 0.0075 g/L vitamin B1 (thiamine HCl), 0.08 g/L tyrosine, 5.0 g/L glucose-H2O, 0.1 g/L ampicillin. The trace element solution consisted of: 2.0 g/L Al2(SO4)3-18H2O, 0.75 g/L CoSO4-7H2O, 2.5 g/L CuSO4-5H2O, 0.5 g/L H3BO4, 24 g/L MnSO4, 3.0 g/L Na2MoO4-2H2O, 2.5 g/L NiSO4-6H2O, 15 g/L ZnSO4-7H2O.
Carbon source Glucose
Growth rate
Case-specific description Monitored are the periods 20.5-23.3h.
Specific Rate (mmol g-1 h-1)
Specific Rate (mmol g-1 h-1)
Specific Rate (mmol g-1 h-1)
Specific Rate (mmol g-1 h-1)
Source