Bioprocess Biosyst Eng. 2013 Sep;36(9):1261-5. Metabolic flux analysis of genetically engineered Saccharomyces cerevisiae that produces lactate under micro-aerobic conditions. Nagamori E, Shimizu K, Fujita H, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 1.34 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0.56 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 1.48 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 0 | R02736+R01528 |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae YIBO-7A |
| Genotype | |
| Culture medium | For cell culture,aerobic cultivation was performed in a mineral medium supplemented with 20 g/L glucose, prepared according to Verduyn et al. The cells were cultivated aerobically in 0.5 L of growth medium, in a 1L bioreactor at 30 Celsius, with agitation at 500 rpm and 0.5 L/min of air aeration (aerobic condition).For micro-aerobic lactate fermentation, harvested cells were inoculated in 0.5 L of the mineral medium supplemented with 100 g/Lglucose with an initial cell concentration of 1.0 g/Ldry cells. The fermentation was performed with an agitation speed of 300 rpm and aeration rate of 0.1 L/min of air (micro-aerobic condition: overall oxygen transfer coefficient was measured to be 18/h by dynamic method). For all cultivations performed, pH was maintained between 4.8 and 5.0 by using 1.2 M NaOH and 1.4 M HCl solutions. |
| Carbon source | Glucose |
| Growth rate | 0.029/h |
| Case-specific description | Estimated carbon flux distributions of metabolically engineered yeast strain YIBO-7A under micro-aerobic conditions. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae T165R |
| Genotype | |
| Culture medium | For cell culture,aerobic cultivation was performed in a mineral medium supplemented with 20 g/L glucose, prepared according to Verduyn et al. The cells were cultivated aerobically in 0.5 L of growth medium, in a 1L bioreactor at 30 Celsius, with agitation at 500 rpm and 0.5 L/min of air aeration (aerobic condition).For micro-aerobic lactate fermentation, harvested cells were inoculated in 0.5 L of the mineral medium supplemented with 100 g/Lglucose with an initial cell concentration of 1.0 g/Ldry cells. The fermentation was performed with an agitation speed of 300 rpm and aeration rate of 0.1 L/min of air (micro-aerobic condition: overall oxygen transfer coefficient was measured to be 18/h by dynamic method). For all cultivations performed, pH was maintained between 4.8 and 5.0 by using 1.2 M NaOH and 1.4 M HCl solutions. |
| Carbon source | Glucose |
| Growth rate | 0.025/h |
| Case-specific description | Estimated carbon flux distributions of metabolically engineered yeast strain T165R under micro-aerobic conditions. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae AF297C |
| Genotype | |
| Culture medium | For cell culture,aerobic cultivation was performed in a mineral medium supplemented with 20 g/L glucose, prepared according to Verduyn et al. The cells were cultivated aerobically in 0.5 L of growth medium, in a 1L bioreactor at 30 Celsius, with agitation at 500 rpm and 0.5 L/min of air aeration (aerobic condition).For micro-aerobic lactate fermentation, harvested cells were inoculated in 0.5 L of the mineral medium supplemented with 100 g/Lglucose with an initial cell concentration of 1.0 g/Ldry cells. The fermentation was performed with an agitation speed of 300 rpm and aeration rate of 0.1 L/min of air (micro-aerobic condition: overall oxygen transfer coefficient was measured to be 18/h by dynamic method). For all cultivations performed, pH was maintained between 4.8 and 5.0 by using 1.2 M NaOH and 1.4 M HCl solutions. |
| Carbon source | Glucose |
| Growth rate | 0.009/h |
| Case-specific description | Estimated carbon flux distributions of metabolically engineered yeast strain AF297C under micro-aerobic conditions. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae YIBO-pdc1/5 delta |
| Genotype | |
| Culture medium | For cell culture,aerobic cultivation was performed in a mineral medium supplemented with 20 g/L glucose, prepared according to Verduyn et al. The cells were cultivated aerobically in 0.5 L of growth medium, in a 1L bioreactor at 30 Celsius, with agitation at 500 rpm and 0.5 L/min of air aeration (aerobic condition).For micro-aerobic lactate fermentation, harvested cells were inoculated in 0.5 L of the mineral medium supplemented with 100 g/Lglucose with an initial cell concentration of 1.0 g/Ldry cells. The fermentation was performed with an agitation speed of 300 rpm and aeration rate of 0.1 L/min of air (micro-aerobic condition: overall oxygen transfer coefficient was measured to be 18/h by dynamic method). For all cultivations performed, pH was maintained between 4.8 and 5.0 by using 1.2 M NaOH and 1.4 M HCl solutions. |
| Carbon source | Glucose |
| Growth rate | 0.007/h |
| Case-specific description | Estimated carbon flux distributions of metabolically engineered yeast strain YIBO-pdc1/5 delta under micro-aerobic conditions. |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |
| Specific Rate | (mmol g-1 h-1) |