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Microb Cell Fact. 2008 Jun;7:19. Maintenance metabolism and carbon fluxes in Bacillus species. Tannler S, Decasper S, Sauer U.



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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 122 R02736+R01528+R00267+R00214
NADPH production rate Minium 64 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 124 R02736+R01528+R00267+R00214
NADPH production rate Minium 64 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 127 R02736+R01528+R00267+R00214
NADPH production rate Minium 64 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 107 R02736+R01528+R00267+R00214
NADPH production rate Minium 76 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 102 R02736+R01528+R00267+R00214
NADPH production rate Minium 68 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 114 R02736+R01528+R00267+R00214
NADPH production rate Minium 78 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 76 R02736+R01528+R00267+R00214
NADPH production rate Minium 44 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 167 R02736+R01528+R00267+R00214
NADPH production rate Minium 60 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 174 R02736+R01528+R00267+R00214
NADPH production rate Minium 38 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 173 R02736+R01528+R00267+R00214
NADPH production rate Minium 58 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 177 R02736+R01528+R00267+R00214
NADPH production rate Minium 56 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 121 R02736+R01528+R00267+R00214
NADPH production rate Minium 52 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 148 R02736+R01528+R00267+R00214
NADPH production rate Minium 52 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum 131 R02736+R01528+R00267+R00214
NADPH production rate Minium 58 R02736+R01528
Growth Condition
Strains Bacillus subtilis wild type 168
Genotype trp+
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.67/h
Case-specific description Relative distribution of absolute fluxes in glucose batch cultures of B.subtilis wild type.
Growth Condition
Strains Bacillus subtilis spo0A
Genotype trp+ spo0A-
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.71/h
Case-specific description Relative distribution of absolute fluxes in glucose batch cultures of B.subtilis spo0A.
Growth Condition
Strains Bacillus subtilis sigE
Genotype trp+ sigE-
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.67/h
Case-specific description Relative distribution of absolute fluxes in glucose batch cultures of B.subtilis sigE.
Growth Condition
Strains Bacillus subtilis natto(DSM No. 4451)
Genotype bio- ade-
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.69/h
Case-specific description Relative distribution of absolute fluxes in glucose batch cultures of B.subtilis natto.
Growth Condition
Strains Bacillus licheniformis T218a
Genotype
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.37/h
Case-specific description Relative distribution of absolute fluxes in glucose batch cultures of B.licheniformis T218a.
Growth Condition
Strains Bacillus licheniformis T380B
Genotype
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.49/h
Case-specific description Relative distribution of absolute fluxes in glucose batch cultures of B.licheniformis T380B.
Growth Condition
Strains Bacillus amyloliquefaciens
Genotype
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.32/h
Case-specific description Relative distribution of absolute fluxes in glucose batch cultures of B.amyloliquefaciens.
Growth Condition
Strains Bacillus pumilus(DSM No.27)
Genotype bio- ade-
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.28/h
Case-specific description Relative distribution of absolute fluxes in glucose batch cultures of B.pumilus.
Growth Condition
Strains Bacillus subtilis wild type 168
Genotype trp+
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.05/h
Case-specific description Relative distribution of absolute fluxes in B.subtilis wild type at dilution rates of 0.05/h.
Growth Condition
Strains Bacillus subtilis wild type 168
Genotype trp+
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.2/h
Case-specific description Relative distribution of absolute fluxes in B.subtilis wild type at dilution rates of 0.2/h.
Growth Condition
Strains Bacillus subtilis wild type 168
Genotype trp+
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.4/h
Case-specific description Relative distribution of absolute fluxes in B.subtilis wild type at dilution rates of 0.4/h.
Growth Condition
Strains Bacillus licheniformis T218a
Genotype
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.2/h
Case-specific description Relative distribution of absolute fluxes in Bacillus licheniformis T218a at dilution rate 0.2/h.
Growth Condition
Strains Bacillus licheniformis T380B
Genotype
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.2/h
Case-specific description Relative distribution of absolute fluxes in Bacillus licheniformis T380B at dilution rate 0.2/h.
Growth Condition
Strains Bacillus subtilis natto(DSM No. 4451)
Genotype bio- ade-
Culture medium The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter.
Carbon source Glucose
Growth rate 0.2/h
Case-specific description Relative distribution of absolute fluxes in Bacillus subtilis natto at dilution rate 0.2/h.
Specific Rate (mmol g-1 h-1)
glucose 8.71
acetate 4.28
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 11.3
acetate 7.02
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 7.9
acetate 3.6
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 10.83
acetate 9.6
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 4.63
acetate 3.03
pyruvate 0.22
Specific Rate (mmol g-1 h-1)
glucose 6.67
acetate 4.17
pyruvate 0.4
Specific Rate (mmol g-1 h-1)
glucose 6.05
acetate N/A
pyruvate 5.45
Specific Rate (mmol g-1 h-1)
glucose 4.17
acetate 1.4
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 0.39
acetate N/A
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 0.49
acetate N/A
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 0.33
acetate N/A
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 0.3
acetate N/A
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 0.2
acetate N/A
pyruvate N/A
Specific Rate (mmol g-1 h-1)
glucose 0.35
acetate N/A
pyruvate N/A
Source