Microb Cell Fact. 2008 Jun;7:19. Maintenance metabolism and carbon fluxes in Bacillus species. Tannler S, Decasper S, Sauer U.
Click on the "GO TO" button to display the flux map.
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 122 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 64 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 124 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 64 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 127 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 64 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 107 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 76 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 102 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 68 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 114 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 78 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 76 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 44 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 167 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 60 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 174 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 38 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 173 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 58 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 177 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 56 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 121 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 52 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 148 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 52 | R02736+R01528 |
ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
NADPH production rate Maximum | 131 | R02736+R01528+R00267+R00214 |
NADPH production rate Minium | 58 | R02736+R01528 |
Growth Condition | |
Strains | Bacillus subtilis wild type 168 |
Genotype | trp+ |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.67/h |
Case-specific description | Relative distribution of absolute fluxes in glucose batch cultures of B.subtilis wild type. |
Growth Condition | |
Strains | Bacillus subtilis spo0A |
Genotype | trp+ spo0A- |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.71/h |
Case-specific description | Relative distribution of absolute fluxes in glucose batch cultures of B.subtilis spo0A. |
Growth Condition | |
Strains | Bacillus subtilis sigE |
Genotype | trp+ sigE- |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.67/h |
Case-specific description | Relative distribution of absolute fluxes in glucose batch cultures of B.subtilis sigE. |
Growth Condition | |
Strains | Bacillus subtilis natto(DSM No. 4451) |
Genotype | bio- ade- |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.69/h |
Case-specific description | Relative distribution of absolute fluxes in glucose batch cultures of B.subtilis natto. |
Growth Condition | |
Strains | Bacillus licheniformis T218a |
Genotype | |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.37/h |
Case-specific description | Relative distribution of absolute fluxes in glucose batch cultures of B.licheniformis T218a. |
Growth Condition | |
Strains | Bacillus licheniformis T380B |
Genotype | |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.49/h |
Case-specific description | Relative distribution of absolute fluxes in glucose batch cultures of B.licheniformis T380B. |
Growth Condition | |
Strains | Bacillus amyloliquefaciens |
Genotype | |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.32/h |
Case-specific description | Relative distribution of absolute fluxes in glucose batch cultures of B.amyloliquefaciens. |
Growth Condition | |
Strains | Bacillus pumilus(DSM No.27) |
Genotype | bio- ade- |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.28/h |
Case-specific description | Relative distribution of absolute fluxes in glucose batch cultures of B.pumilus. |
Growth Condition | |
Strains | Bacillus subtilis wild type 168 |
Genotype | trp+ |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.05/h |
Case-specific description | Relative distribution of absolute fluxes in B.subtilis wild type at dilution rates of 0.05/h. |
Growth Condition | |
Strains | Bacillus subtilis wild type 168 |
Genotype | trp+ |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.2/h |
Case-specific description | Relative distribution of absolute fluxes in B.subtilis wild type at dilution rates of 0.2/h. |
Growth Condition | |
Strains | Bacillus subtilis wild type 168 |
Genotype | trp+ |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.4/h |
Case-specific description | Relative distribution of absolute fluxes in B.subtilis wild type at dilution rates of 0.4/h. |
Growth Condition | |
Strains | Bacillus licheniformis T218a |
Genotype | |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.2/h |
Case-specific description | Relative distribution of absolute fluxes in Bacillus licheniformis T218a at dilution rate 0.2/h. |
Growth Condition | |
Strains | Bacillus licheniformis T380B |
Genotype | |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.2/h |
Case-specific description | Relative distribution of absolute fluxes in Bacillus licheniformis T380B at dilution rate 0.2/h. |
Growth Condition | |
Strains | Bacillus subtilis natto(DSM No. 4451) |
Genotype | bio- ade- |
Culture medium | The M9 minimal medium was composed of the following components (per liter final volume): 5.64 g Na2HPO4, 3 g KH2PO4, 0.5 g NaCl, 1 g NH4Cl, 0.246 g MgSO4-7H2O, 0.014 g CaCl2, at pH 7.4 and 10 ml trace element solution containing (per liter) 1.35 g FeCl2-6H2O, 0.1 g MnCl2-H2O, 0.17 g ZnCl2, 0.043 g CuCl2-2H2O, 0.06 g CoCl2-6H2O, 0.06 g Na2MoO4-2H2O.Filter-sterilized glucose was added to a final concentration of 1 g per liter. |
Carbon source | Glucose |
Growth rate | 0.2/h |
Case-specific description | Relative distribution of absolute fluxes in Bacillus subtilis natto at dilution rate 0.2/h. |
Specific Rate | (mmol g-1 h-1) |
glucose | 8.71 |
acetate | 4.28 |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 11.3 |
acetate | 7.02 |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 7.9 |
acetate | 3.6 |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 10.83 |
acetate | 9.6 |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 4.63 |
acetate | 3.03 |
pyruvate | 0.22 |
Specific Rate | (mmol g-1 h-1) |
glucose | 6.67 |
acetate | 4.17 |
pyruvate | 0.4 |
Specific Rate | (mmol g-1 h-1) |
glucose | 6.05 |
acetate | N/A |
pyruvate | 5.45 |
Specific Rate | (mmol g-1 h-1) |
glucose | 4.17 |
acetate | 1.4 |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 0.39 |
acetate | N/A |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 0.49 |
acetate | N/A |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 0.33 |
acetate | N/A |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 0.3 |
acetate | N/A |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 0.2 |
acetate | N/A |
pyruvate | N/A |
Specific Rate | (mmol g-1 h-1) |
glucose | 0.35 |
acetate | N/A |
pyruvate | N/A |