Microb Cell Fact. 2008 Mar;7:8. Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum. Becker J, Klopprogge C, Wittmann C.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Corynebacterium glutamicum lysCfbr |
| Genotype | lysCfbr |
| Culture medium | Complex medium containing 5g/L glucose,5g/L yeast extract,10g/L tryptone,5g/L NaCl and 18 g/L agar was used for agar plates.First pre-cultures were grown in the same medium without agar.Second pre-cultivation and main cultivation was performed in minimal medium with 80mM glucose as carbon source. The minimal medium additionally contained per liter:0.055g CaCl2-2H2O,0.2g MgSO4-7H2O,1.0g NaCl,16.0g K2HPO4,2.0g KH2PO4,5.0g (NH4)2SO4,0.5mg biotin,1mg Ca-panthothenic acid,1mg thiamine-HCl,20mg FeSO4,30mg 3,4-dihydroxybenzoic acid and 10ml of trace element solution. |
| Carbon source | Glucose. |
| Growth rate | 0.38/h |
| Case-specific description | In vivo carbon flux distribution in the central metabolism of lysine producing C.glutamicum lysCfbr during growth on glucose. |
| Growth Condition | |
| Strains | Corynebacterium glutamicum lysCfbr delta pyk |
| Genotype | lysCfbr delta pyk |
| Culture medium | Complex medium containing 5g/L glucose,5g/L yeast extract,10g/L tryptone,5g/L NaCl and 18 g/L agar was used for agar plates.First pre-cultures were grown in the same medium without agar.Second pre-cultivation and main cultivation was performed in minimal medium with 80mM glucose as carbon source. The minimal medium additionally contained per liter:0.055g CaCl2-2H2O,0.2g MgSO4-7H2O,1.0g NaCl,16.0g K2HPO4,2.0g KH2PO4,5.0g (NH4)2SO4,0.5mg biotin,1mg Ca-panthothenic acid,1mg thiamine-HCl,20mg FeSO4,30mg 3,4-dihydroxybenzoic acid and 10ml of trace element solution. |
| Carbon source | Glucose. |
| Growth rate | 0.35/h |
| Case-specific description | In vivo carbon flux distribution in the central metabolism of pyruvate kinase deficient derivative C.glutamicum lysCfbr delta pyk during growth on glucose. |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.6 |
| lysine | 0.38 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 4.5 |
| lysine | 0.33 |