Biotechnol Bioeng. 2008 Apr;99(5):1170-85. Metabolic flux analysis in Escherichia coli by integrating isotopic dynamic and isotopic stationary 13C labeling data. Schaub J, Mauch K, Reuss M.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | N/A | R02736+R01528 |
| Growth Condition | |
| Strains | Escherichia coli K12 W3110 |
| Genotype | |
| Culture medium | M9 minimal medium contained concentrations (per liter deionized) as follows: Na2HPO4-2H2O 7.5 g, KH2PO4 3.0 g, NaCl 0.5 g, NH4Cl 2.7 g, CaCl2 11 mg, MgSO4 0.24 g; and 1 mL of a trace element solution per liter mineral medium according to (perliter bidistilled water): FeCl3-6H2O 16.7 g, ZnSO4-7H2O 0.18 g, CuCl2-2H2O 0.12 g, MnSO4-H2O 0.12 g, CoCl2-6H2O 0.18 g, Na2EDTA-2H2O 22.25 g. Glucose concentration in the medium was 5.0 g/L. The medium was acidified by addition of 14 mL H2SO4 (95%) to a pH between 2 and 3, and sterilized by filtration. |
| Carbon source | Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Steady state flux distribution in glycolysis, PPP, and TCA in E.coli at D=0.10/h as identified by ID-13C MFA. |
| Growth Condition | |
| Strains | Escherichia coli K12 W3110 |
| Genotype | |
| Culture medium | M9 minimal medium contained concentrations (per liter deionized) as follows: Na2HPO4-2H2O 7.5 g, KH2PO4 3.0 g, NaCl 0.5 g, NH4Cl 2.7 g, CaCl2 11 mg, MgSO4 0.24 g; and 1 mL of a trace element solution per liter mineral medium according to (perliter bidistilled water): FeCl3-6H2O 16.7 g, ZnSO4-7H2O 0.18 g, CuCl2-2H2O 0.12 g, MnSO4-H2O 0.12 g, CoCl2-6H2O 0.18 g, Na2EDTA-2H2O 22.25 g. Glucose concentration in the medium was 5.0 g/L. The medium was acidified by addition of 14 mL H2SO4 (95%) to a pH between 2 and 3, and sterilized by filtration. |
| Carbon source | Glucose |
| Growth rate | 0.1/h |
| Case-specific description | Steady state flux distribution in glycolysis, PPP, and TCA in E.coli at D=0.10/h as identified by IS-13C MFA |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 6.76 |
| CO2 | 2.57 |
| Specific Rate | (mmol g-1 h-1) |
| glucose | 6.76 |
| CO2 | 2.57 |