Metab Eng. 2003 Jan;5(1):16-31. Metabolic flux analysis of xylose metabolism in recombinant Saccharomyces cerevisiae using continuous culture. Pitkanen JP, Aristidou A, Salusjarvi L, et al.
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| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 12 | R02736+R01528 |
| ATP production rate Maximum | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342 +2.5*R00214 |
| ATP production rate Minium | N/A | -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209 +2.5*R08549-R00405+1.5*R00402+2.5*R00342 |
| NADPH production rate Maximum | N/A | R02736+R01528+R00267+R00214 |
| NADPH production rate Minium | 98 | R02736+R01528 |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae H2466 |
| Genotype | |
| Culture medium | The liquid growth media contained 6.7 g/L yeast nitrogen base (YNB) without amino acids and D-glucose. Concentrated sugar solutions were sterilized separately in an autoclave. The amino acids required for strain H2466 (L-histidine 0.058 g/L and L-tryptophan 0.082 g/L Fluka) were filter-sterilized through a 0.22-mm cellulose-acetate filter. YNB solutions were sterilized by autoclaving for the chemostat cultures, but otherwise were filter-sterilized. Media for chemostat cultivations also contained 0.5 ml/L silicone antifoam. |
| Carbon source | Glucose(10 g/L) |
| Growth rate | 0.05/h |
| Case-specific description | The central carbon fluxes under aerobic conditions on glucose (10g/L) in continuous chemostat cultivations at the dilution rate of 0.05/h, and the specific glucose flux in was 1.20mmol/h/g. The metabolic network was based on the one described by Nissen and coworkers (Nissen et al., 1997), with a number of modifications including the addition of the xyloseutilizing reactions. |
| Growth Condition | |
| Strains | Saccharomyces cerevisiae H2466 |
| Genotype | |
| Culture medium | The liquid growth media contained 6.7 g/L yeast nitrogen base (YNB) without amino acids and a mixture of D-glucose and D-xylose. Concentrated sugar solutions were sterilized separately in an autoclave. The amino acids required for strain H2466 (L-histidine 0.058 g/L and L-tryptophan 0.082 g/L Fluka) were filter-sterilized through a 0.22-mm cellulose-acetate filter. YNB solutions were sterilized by autoclaving for the chemostat cultures, but otherwise were filter-sterilized. Media for chemostat cultivations also contained 0.5 ml/L silicone antifoam. |
| Carbon source | Xylose-glucose mixture(27:3 g/L) |
| Growth rate | 0.05/h |
| Case-specific description | The central carbon fluxes under aerobic conditions on xylose–glucose mixture (27:3g/L) in continuous chemostat cultivations at the dilution rate of 0.05/h, and glucose flux in was 0.32mmol/h/g and the xylose flux in 0.72mmol/h/g. The metabolic network was based on the one described by Nissen and coworkers (Nissen et al., 1997), with a number of modifications including the addition of the xyloseutilizing reactions. |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 1.2 |
| Xylose | N/A |
| Specific Rate | (mmol g-1 h-1) |
| Glucose | 0.32 |
| Xylose | 0.72 |