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Metab Eng. 2003 Jan;5(1):16-31. Metabolic flux analysis of xylose metabolism in recombinant Saccharomyces cerevisiae using continuous culture. Pitkanen JP, Aristidou A, Salusjarvi L, et al.



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ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 928 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 12 R02736+R01528
ATP production rate Maximum N/A -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R00267+2.5*R08549-R00405+1.5*R00402+2.5*R00342
+2.5*R00214
ATP production rate Minium 624.5 -R01786-R04779+2.5*R01061+R01512+R00200+2.5*R00209
+2.5*R08549-R00405+1.5*R00402+2.5*R00342
NADPH production rate Maximum N/A R02736+R01528+R00267+R00214
NADPH production rate Minium 98 R02736+R01528
Growth Condition
Strains Saccharomyces cerevisiae H2466
Genotype
Culture medium The liquid growth media contained 6.7 g/L yeast nitrogen base (YNB) without amino acids and D-glucose. Concentrated sugar solutions were sterilized separately in an autoclave. The amino acids required for strain H2466 (L-histidine 0.058 g/L and L-tryptophan 0.082 g/L Fluka) were filter-sterilized through a 0.22-mm cellulose-acetate filter. YNB solutions were sterilized by autoclaving for the chemostat cultures, but otherwise were filter-sterilized. Media for chemostat cultivations also contained 0.5 ml/L silicone antifoam.
Carbon source Glucose(10 g/L)
Growth rate 0.05/h
Case-specific description The central carbon fluxes under aerobic conditions on glucose (10g/L) in continuous chemostat cultivations at the dilution rate of 0.05/h, and the specific glucose flux in was 1.20mmol/h/g. The metabolic network was based on the one described by Nissen and coworkers (Nissen et al., 1997), with a number of modifications including the addition of the xyloseutilizing reactions.
Growth Condition
Strains Saccharomyces cerevisiae H2466
Genotype
Culture medium The liquid growth media contained 6.7 g/L yeast nitrogen base (YNB) without amino acids and a mixture of D-glucose and D-xylose. Concentrated sugar solutions were sterilized separately in an autoclave. The amino acids required for strain H2466 (L-histidine 0.058 g/L and L-tryptophan 0.082 g/L Fluka) were filter-sterilized through a 0.22-mm cellulose-acetate filter. YNB solutions were sterilized by autoclaving for the chemostat cultures, but otherwise were filter-sterilized. Media for chemostat cultivations also contained 0.5 ml/L silicone antifoam.
Carbon source Xylose-glucose mixture(27:3 g/L)
Growth rate 0.05/h
Case-specific description The central carbon fluxes under aerobic conditions on xylose–glucose mixture (27:3g/L) in continuous chemostat cultivations at the dilution rate of 0.05/h, and glucose flux in was 0.32mmol/h/g and the xylose flux in 0.72mmol/h/g. The metabolic network was based on the one described by Nissen and coworkers (Nissen et al., 1997), with a number of modifications including the addition of the xyloseutilizing reactions.
Specific Rate (mmol g-1 h-1)
Glucose 1.2
Xylose N/A
Specific Rate (mmol g-1 h-1)
Glucose 0.32
Xylose 0.72
Source